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绘制你的检测方法:通过在纸张上绘图制作低成本的纸质诊断和多孔测试区域。

Draw your assay: Fabrication of low-cost paper-based diagnostic and multi-well test zones by drawing on a paper.

作者信息

Oyola-Reynoso Stephanie, Heim Andrew P, Halbertsma-Black Julian, Zhao C, Tevis Ian D, Çınar Simge, Cademartiri Rebecca, Liu Xinyu, Bloch Jean-Francis, Thuo Martin M

机构信息

Department of Materials Science and Engineering, Iowa State University, Ames, IA 50011, USA.

Department of Chemistry, University of Massachusetts Boston, Boston, MA 02138, USA.

出版信息

Talanta. 2015 Nov 1;144:289-93. doi: 10.1016/j.talanta.2015.06.018. Epub 2015 Jun 11.

Abstract

Interest in low-cost diagnostic devices has recently gained attention, in part due to the rising cost of healthcare and the need to serve populations in resource-limited settings. A major challenge in the development of such devices is the need for hydrophobic barriers to contain polar bio-fluid analytes. Key approaches in lowering the cost in diagnostics have centered on (i) development of low-cost fabrication techniques/processes, (ii) use of affordable materials, or, (iii) minimizing the need for high-tech tools. This communication describes a simple, low-cost, adaptable, and portable method for patterning paper and subsequent use of the patterned paper in diagnostic tests. Our approach generates hydrophobic regions using a ball-point pen filled with a hydrophobizing molecule suspended in a solvent carrier. An empty ball-point pen was filled with a solution of trichloro perfluoroalkyl silane in hexanes (or hexadecane), and the pen used to draw lines on Whatman® chromatography 1 paper. The drawn regions defined the test zones since the trichloro silane reacts with the paper to give a hydrophobic barrier. The formation of the hydrophobic barriers is reaction kinetic and diffusion-limited, ensuring well defined narrow barriers. We performed colorimetric glucose assays and enzyme-linked immuno-sorbent assay (ELISA) using the created test zones. To demonstrate the versatility of this approach, we fabricated multiple devices on a single piece of paper and demonstrated the reproducibility of assays on these devices. The overall cost of devices fabricated by drawing are relatively lower (<US $0.001 per device) than those derived from wax-printing (US$0.05-0.003) or other approaches.

摘要

对低成本诊断设备的兴趣近来受到关注,部分原因是医疗保健成本不断上升以及需要为资源有限地区的人群提供服务。开发此类设备的一个主要挑战是需要疏水屏障来容纳极性生物流体分析物。降低诊断成本的关键方法集中在:(i)开发低成本制造技术/工艺;(ii)使用价格合理的材料;或(iii)尽量减少对高科技工具的需求。本通讯介绍了一种简单、低成本、适应性强且便于携带的方法,用于在纸张上进行图案化处理,以及随后在诊断测试中使用图案化纸张。我们的方法是使用一支装有悬浮在溶剂载体中的疏水化分子的圆珠笔来生成疏水区域。将一支空圆珠笔装满三氯全氟烷基硅烷的己烷(或十六烷)溶液,然后用这支笔在沃特曼®1号层析纸上划线。由于三氯硅烷与纸张发生反应形成疏水屏障,所划区域就定义了测试区域。疏水屏障的形成受反应动力学和扩散限制,确保形成界限分明的狭窄屏障。我们使用创建的测试区域进行了比色葡萄糖测定和酶联免疫吸附测定(ELISA)。为了证明这种方法的通用性,我们在一张纸上制作了多个设备,并展示了这些设备上测定的可重复性。通过划线制作的设备总成本(每个设备<0.001美元)相对低于蜡印法(0.05 - 0.03美元)或其他方法制作的设备。

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