Boudoukha Chahra, Bouriche Hamama, Ortega Eduardo, Senator Abderrahmane
a Laboratory of Applied Biochemistry , Faculty of Nature and Life Sciences, University of Ferhat Abbas , Setif , Algeria and.
b Group of Immunophysiology, Department of Physiology, Faculty of Sciences, University of Extremadura , Badajoz , Spain.
Pharm Biol. 2016;54(4):667-73. doi: 10.3109/13880209.2015.1071853. Epub 2015 Oct 9.
Santolina chamaecyparissus L. (Asteraceae) is an aromatic plant wide spread in the Mediterranean region. It is used in folk medicine for its anti-inflammatory properties.
The effects of S. chamaecyparissus aqueous extract (SCAE) and polyphenolic extract (SCPE) on human polymorphonuclear neutrophil (PMN) degranulation, chemotaxis, phagocytosis, and microbicidal capacity were examined in vitro.
Aqueous and polyphenolic extracts were prepared from S. chamaecyparissus leaves. The elastase release was used as a marker for measuring PMN degranulation, while chemotaxis was performed using a 48-microwell chemotaxis chamber. The phagocytosis and the microbicidal capacity were evaluated using fresh cultures of Candida albicans.
The treatment of neutrophils with different concentrations (10-200 µg/ml) of SCAE and SCPE caused a significant (p < 0.001) and dose-dependent inhibitory effect on elastase release in fMLP/Cytochalasin B (CB)-stimulated neutrophils. Indeed, 100 µg/ml of SCAE exerted an inhibitory effect of 51.97 ± 6.2%, whereas SCPE at the same concentration abolished completely PMN degranulation. Moreover, both extracts inhibited markedly (p < 0.01) fMLP-induced chemotactic migration. At 200 µg/ml, SCAE and SCPE exerted an inhibitory effect of 54.61 ± 7.3% and 57.71 ± 7.44%, respectively. In addition, a decline in both phagocytosis and microbicidal capacity against Candida albicans was observed when PMNs were exposed to 100 and 200 µg/ml of SCAE or SCPE.
The exerted effects on neutrophil functions support the anti-inflammatory activity and show new mechanisms of action and effectiveness of S. chamaecyparissus leaf extracts. This plant may be considered as an interesting source of anti-inflammatory and immunomodulatory agents.
圣托里尼柏（菊科）是一种在地中海地区广泛分布的芳香植物。它因其抗炎特性而被用于民间医学。
体外研究圣托里尼柏水提取物（SCAE）和多酚提取物（SCPE）对人多形核中性粒细胞（PMN）脱颗粒、趋化性、吞噬作用和杀菌能力的影响。
从圣托里尼柏叶片中制备水提取物和多酚提取物。弹性蛋白酶释放用作测量PMN脱颗粒的标志物，而趋化性则使用48孔趋化性小室进行检测。使用白色念珠菌的新鲜培养物评估吞噬作用和杀菌能力。
用不同浓度（10 - 200μg/ml）的SCAE和SCPE处理中性粒细胞，对fMLP/细胞松弛素B（CB）刺激的中性粒细胞中的弹性蛋白酶释放产生了显著（p < 0.001）且剂量依赖性的抑制作用。实际上，100μg/ml的SCAE产生了51.97±6.2%的抑制作用，而相同浓度的SCPE则完全消除了PMN脱颗粒。此外，两种提取物均显著（p < 0.01）抑制了fMLP诱导的趋化迁移。在200μg/ml时，SCAE和SCPE的抑制作用分别为54.61±7.3%和57.71±7.44%。此外，当中性粒细胞暴露于100和200μg/ml的SCAE或SCPE时，观察到对白色念珠菌的吞噬作用和杀菌能力均有所下降。
对中性粒细胞功能的作用支持了其抗炎活性，并显示了圣托里尼柏叶提取物新的作用机制和有效性。这种植物可被视为一种有趣的抗炎和免疫调节剂来源。
