School of Chemistry and Environment Science, Beijing University of Aeronautics and Astronautics , Beijing 100191, China.
ACS Appl Mater Interfaces. 2015 Oct 28;7(42):23418-22. doi: 10.1021/acsami.5b08924. Epub 2015 Oct 15.
A new liquid-crystal (LC)-based sensor operated by nickel nanosphere (NiNS)-induced homeotropic alignment for the label-free monitoring of thrombin was reported. When doped with NiNSs, a uniform vertical orientation of 4-cyano-4'-pentylbiphenyl (5CB) was easily obtained. A sandwich system of aptamer/thrombin/aptamer-functionalized gold nanoparticles (AuNPs) was fabricated, and AuNPs-aptamer conjugation caused the disruption of the 5CB orientation, leading to an obvious change of the optical appearance from a dark to a bright response to thrombin concentrations from 0.1 to 100 nM. This design also allowed quantitative detection of the thrombin concentration. This distinctive and sensitive thrombin LC sensor provides a new principle for building LC-sensing systems.
一种基于镍纳米球（NiNS）诱导的各向异性排列的新型液晶（LC）传感器，可用于无标记监测凝血酶。当掺杂 NiNS 时，很容易获得 4-氰基-4'-戊基联苯（5CB）的均匀垂直取向。制备了适体/凝血酶/适体功能化金纳米粒子（AuNPs）的三明治体系，AuNPs-适体结合导致 5CB 取向的破坏，导致光学外观从黑暗到明亮响应明显变化，对凝血酶浓度从 0.1 到 100 nM 的响应。该设计还允许对凝血酶浓度进行定量检测。这种独特且灵敏的凝血酶 LC 传感器为构建 LC 传感系统提供了新的原理。
