College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
Talanta. 2018 Aug 15;186:60-64. doi: 10.1016/j.talanta.2018.04.004. Epub 2018 Apr 4.
A label-free liquid crystal (LC) biosensor based on orientation changes of LC molecules was reported for the detection of cecropin B. The homeotropic-to-tilted alignment transition of LC molecules, induced by the specific binding event between cecropin B and anti-cecropin B antibody immobilized via glutaraldehyde, could result in obvious change of the optical appearance from a dark to a bright response and as a result, the detection limit of cecropin B was as low as 50 ng/mL. The average gray-scale intensities (GIs) of optical appearances were calculated to quantitatively analyse cecropin B concentrations. This study offers a simple, highly sensitive and specific, lable-free method for cecropin B detection.
一种基于液晶分子取向变化的无标记液晶（LC）生物传感器被报道用于检测抗菌肽 B。通过戊二醛固定的抗菌肽 B 和抗抗菌肽 B 抗体之间的特异性结合事件诱导 LC 分子的近晶相到倾斜排列的转变，会导致光学外观从暗到亮的明显变化，从而使抗菌肽 B 的检测限低至 50ng/mL。通过计算光学外观的平均灰度强度（GI）来定量分析抗菌肽 B 的浓度。该研究提供了一种简单、高灵敏度和特异性的无标记方法来检测抗菌肽 B。
