Reuter Cézane Priscila, Rosane De Moura Valim Andréia, Gaya Anelise Reis, Borges Tássia Silvana, Klinger Elisa Inês, Possuelo Lia Gonçalves, Franke Silvia Isabel Rech, Kmetzsch Lívia, Vainstein Marilene Henning, Prá Daniel, Burgos Miria Suzana
Department of Physical Education and Health, Postgraduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, RS, 96815-900, Brazil.
Department of Biology and Pharmacy, Postgraduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, RS, 96815-900, Brazil.
Am J Hum Biol. 2016 May;28(3):381-6. doi: 10.1002/ajhb.22798. Epub 2015 Oct 12.
The aim of the present study was to evaluate the relationship between the rs9939609 fat mass and obesity-associated (FTO) polymorphism and cardiorespiratory fitness (CRF) with overweight/obesity outcomes in youth.
This study included 420 youths, comprising 211 boys and 209 girls aged 7-17. Overweight/obesity were evaluated by body mass index (BMI), waist circumference (WC), and the percentage of fat (PF) according to two skinfold thickness measurements. Genotyping of the rs9939609 polymorphism was conducted using real-time Polymerase Chain Reaction (PCR) utilizing TaqMan(®) probes, and CRF was evaluated through a 9-minute run/walk test, categorized as fit or unfit. Logistic regression was utilized to evaluate a possible association between the polymorphism and CRF, with three obesity indicators evaluated.
Individuals with the genotype risk (AA) of FTO polymorphism rs9939609 showed higher prevalence of overweight/obesity, as evaluated by BMI (OR: 3.21; CI: 1.71-6.05), WC (OR: 2.59; CI: 1.35-4.97), and PF (OR: 2.59; CI: 1.36-4.92). Additionally, students with the AA genotype in the unfit model had a significant odds ratio for obesity (OR: 4.40; CI: 1.83-10.61 for BMI; OR: 3.54; CI: 1.58-7.96 for WC), whereas we did not observe associations between the AA genotype with BMI and WC using the fit model. Conversely, PF was associated with the AA genotype only in the fit model (OR: 3.24; CI: 1.26-8.34).
This study demonstrated that the rs9939609 (FTO) polymorphism showed a relationship with obesity in the population studied and an interaction with CRF. Students with low levels of CRF and the AA genotype have a higher risk of being overweight/obese. This association was not found in students with higher levels of CRF. Am. J. Hum. Biol. 28:381-386, 2016. © 2015 Wiley Periodicals, Inc.
本研究旨在评估脂肪量和肥胖相关基因（FTO）rs9939609多态性与青少年心肺适能（CRF）之间的关系及其与超重/肥胖结果的关联。
本研究纳入了420名青少年，其中包括211名男孩和209名女孩，年龄在7至17岁之间。根据两次皮褶厚度测量结果，采用体重指数（BMI）、腰围（WC）和脂肪百分比（PF）评估超重/肥胖情况。利用TaqMan®探针通过实时聚合酶链反应（PCR）对rs9939609多态性进行基因分型，并通过9分钟跑/走测试评估CRF，分为适能或不适能。采用逻辑回归评估该多态性与CRF之间的可能关联，并对三项肥胖指标进行评估。
FTO多态性rs9939609的基因型风险（AA）个体超重/肥胖的患病率较高，通过BMI评估（比值比：3.21；可信区间：1.71 - 6.05）、WC评估（比值比：2.59；可信区间：1.35 - 4.97）和PF评估（比值比：2.59；可信区间：1.36 - 4.92）。此外，在不适能模型中，AA基因型的学生肥胖的比值比显著（BMI的比值比：4.40；可信区间：1.83 - 10.61；WC的比值比：3.54；可信区间：1.58 - 7.96），而在适能模型中，未观察到AA基因型与BMI和WC之间的关联。相反，PF仅在适能模型中与AA基因型相关（比值比：3.24；可信区间：1.26 - 8.34）。
本研究表明，rs9939609（FTO）多态性在所研究人群中与肥胖相关，并与CRF存在相互作用。CRF水平低且具有AA基因型的学生超重/肥胖风险更高。在CRF水平较高的学生中未发现这种关联。《美国人类生物学杂志》2016年第28卷：381 - 386页。©2015威利期刊公司
