Vlach J, Dvorak M, Bartunek P, Pecenka V, Travnicek M, Sponar J
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague.
Biochem Biophys Res Commun. 1989 Feb 15;158(3):737-42. doi: 10.1016/0006-291x(89)92783-6.
We have analyzed the recently sequenced and characterized 2.9 kb fragment derived from the 5'-end of chicken myb proto-oncogene with respect to structural perturbations induced by DNA supercoiling. Within the first intron a 50 bp sequence stretch was localized, starting approximately 450 nucleotides downstream from putative ATG initiation codon, which forms a non-B-DNA structure. Fine mapping with structural probes revealed the three adjacent regions with imperfect purine-pyrimidine alternation creating together relatively long Z-forming tract, parts of which may undergo a B-Z DNA transition at different superhelical densities.
我们分析了最近测序并表征的源自鸡myb原癌基因5'端的2.9 kb片段，研究其由DNA超螺旋引起的结构扰动。在第一个内含子内定位到一段50 bp的序列延伸，起始于假定的ATG起始密码子下游约450个核苷酸处，该序列形成一种非B-DNA结构。用结构探针进行精细定位揭示了三个相邻区域，它们具有不完全的嘌呤-嘧啶交替，共同形成了相对较长的Z型形成区，其中部分区域可能在不同的超螺旋密度下发生B-Z DNA转变。
