Hirano T, Miyajima H, Watanabe T, Tsukuda R, Shimamoto K
J Natl Cancer Inst. 1977 Jan;58(1):73-82. doi: 10.1093/jnci/58.1.73.
Undiluted, fivefold-diluted, and 25-fold-diluted doses of a stock of Moloney murine sarcoma virus were injected directly, in a volume of 0.025 ml, into the backs of fetal Sprague-Dawley rats by laparotomy through the uterine wall at 18 days of gestation. During the first 8 weeks after birth the young responded to the virus with remarkably high but dose-dependent incidences of neoplasms. When a one-fifth dilution of the virus preparation was inoculated at fetal ages 16, 18, and 20 days, the incidences of lesions decreased with advancing fetal age. The tumors developed preferentially at the virus inoculation site and/or in the proximal parts of the extremeties; all were considered to be of mesenchymal derivation, i.e., malignant mesenchymoma, rhabdomyosarcoma, osteosarcoma, fibrosarcoma or fibromyxosarcoma, hemangiosarcoma, plasmacytoma, and a giant cell tumor. This injection procedure provided us with a valuable experimental tool for the rapid screening or testing of potential chemical carcinogens and other biologic studies.
将莫洛尼鼠肉瘤病毒原液、五倍稀释液和二十五倍稀释液，以0.025毫升的体积，在妊娠18天时通过子宫壁剖腹术直接注射到胎龄Sprague-Dawley大鼠的背部。在出生后的前8周，幼鼠对病毒的反应是肿瘤发生率极高，但与剂量有关。当在胎龄16、18和20天时接种病毒制剂的五分之一稀释液时，病变发生率随胎龄增加而降低。肿瘤优先在病毒接种部位和/或四肢近端发展；所有肿瘤均被认为来源于间充质，即恶性间充质瘤、横纹肌肉瘤、骨肉瘤、纤维肉瘤或纤维黏液肉瘤、血管肉瘤、浆细胞瘤和巨细胞瘤。这种注射方法为我们提供了一种有价值的实验工具，可以快速筛选或测试潜在的化学致癌物以及进行其他生物学研究。
