一种来自嗜冷细菌嗜纤维菌IC166(T)的耐冷、耐热且耐变性剂的GH5内切葡聚糖酶Celal_2753的表达与特性鉴定，该酶能耐煮沸。
Expression and characterization of a cold-adapted, thermotolerant and denaturant-stable GH5 endoglucanase Celal_2753 that withstands boiling from the psychrophilic bacterium Cellulophaga algicola IC166(T).
作者信息
Wang Ying, Yu Wengong, Han Feng
机构信息
Key Laboratory of Marine Drugs, Chinese Ministry of Education, 5 Yushan Road, Qingdao, 266003, China.
Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology, 5 Yushan Road, Qingdao, 266003, China.
出版信息
Biotechnol Lett. 2016 Feb;38(2):285-90. doi: 10.1007/s10529-015-1971-5. Epub 2015 Oct 13.
OBJECTIVES
To characterize a novel endoglucanase, Celal_2753, from the psychrophilic bacterium Cellulophaga algicola IC166(T).
RESULTS
Celal_2753 was purified to homogeneity with a yield of 81 % and with a molecular weight of 40 kDa on SDS-PAGE. It had maximum hydrolytic activity towards carboxymethyl cellulose at 40 °C and pH 6. It showed 33 % of the maximum activity at 10 ºC. Its activity increased to 272-316 % in the presence of 0.25-2 M NaCl and KCl at 40 °C. Celal_2753 was stable in the presence of 10 % (v/v) Tween 20, 10 % (v/v) Triton X-100, 16 mM SDS, 6 M urea or 2 M guanidine hydrochloride. Celal_2753 that had been boiled for 5 min recovered 55 % of its initial activity by incubating at 30 °C for 60 min.
CONCLUSION
Because of its cold-adapted, thermotolerant and denaturant-stable properties, endoglucanase Celal_2753 is promising in detergent industry and bioethanol production.
目的
对来自嗜冷细菌食纤维菌属嗜冷栖热菌IC166(T)的一种新型内切葡聚糖酶Celal_2753进行特性分析。
结果
Celal_2753被纯化至同质，产率为81%，在SDS-PAGE上的分子量为40 kDa。它在40℃和pH 6时对羧甲基纤维素具有最大水解活性。在10℃时，它显示出最大活性的33%。在40℃下，0.25 - 2 M的NaCl和KCl存在时，其活性增加到272 - 316%。Celal_2753在10%（v/v）吐温20、10%（v/v）曲拉通X - 100、16 mM SDS、6 M尿素或2 M盐酸胍存在时稳定。煮沸5分钟的Celal_2753在30℃孵育60分钟后恢复了其初始活性的55%。
结论
由于其冷适应、耐热和耐变性的特性，内切葡聚糖酶Celal_2753在洗涤剂工业和生物乙醇生产中具有应用前景。
Zotero 插件