Zhao Ming Xu, Zhou Guo Yu, Zhu Jing Yuan, Gong Biao, Hou Jia Xiang, Zhou Tong, Duan Li Ju, Ding Zhong, Cui Liu Xin, Ba Yue
Department of Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China.
Department of Endemic Disease, Kaifeng Center for Disease Control and Prevention, Kaifeng 475000, Henan, China.
Biomed Environ Sci. 2015 Sep;28(9):696-700. doi: 10.3967/bes2015.099.
The effects of fluoride exposure on the functions of reproductive and endocrine systems have attracted widespread attention in academic circle nowadays. However, it is unclear whether the gene-environment interaction may modify the secretion and activity of hypothalamus-pituitary- ovarian (HPO) axis hormones. Thus, the aim of this study was to explore the influence of fluoride exposure and follicle stimulating hormone receptor (FSHR) gene polymorphism on reproductive hormones in Chinese women. A cross sectional study was conducted in seven villages of Henan Province, China during 2010-2011. A total of 679 women aged 18-48 years were recruited through cluster sampling and divided into three groups, i.e. endemic fluorosis group (EFG), defluoridation project group (DFPG), and control group (CG) based on the local fluoride concentration in drinking water. The serum levels of gonadotropin releasing hormone (GnRH), follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) were determined respectively and the FSHR polymorphism was detected by real time PCR assay. The results provided the preliminary evidence indicating the gene-environment interaction on HPO axis hormones in women.
目前，氟暴露对生殖和内分泌系统功能的影响在学术界引起了广泛关注。然而，基因 - 环境相互作用是否会改变下丘脑 - 垂体 - 卵巢（HPO）轴激素的分泌和活性尚不清楚。因此，本研究的目的是探讨氟暴露和促卵泡激素受体（FSHR）基因多态性对中国女性生殖激素的影响。2010 - 2011年期间，在中国河南省的7个村庄进行了一项横断面研究。通过整群抽样招募了679名年龄在18 - 48岁之间的女性，并根据当地饮用水中的氟浓度将她们分为三组，即地方性氟中毒组（EFG）、改水工程组（DFPG）和对照组（CG）。分别测定血清促性腺激素释放激素（GnRH）、促卵泡激素（FSH）、黄体生成素（LH）和雌二醇（E2）水平，并通过实时PCR测定法检测FSHR基因多态性。结果提供了初步证据，表明女性HPO轴激素存在基因 - 环境相互作用。
