Olukunle O F, Babajide O, Boboye B
Department of Microbiology, Federal University of Technology Akure, P.M.B. 704, Akure, Nigeria.
Open Microbiol J. 2015 Jul 31;9:84-90. doi: 10.2174/1874285801509010084. eCollection 2015.
Enrichment technique was employed for the isolation of the crude oil degrading bacteria. The isolated bacteria were screened for their degradative ability and the best degrading bacteria were selected based on their growth. Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed. Bacteria isolated from the soil sample include; Bacillus cereus, B. amyloliquficiens, B. firmus, Acinetobacter calcoaceticus, Pseudomonas sp. P. fluorescens, P.putida, P.aeruginosa, Achromobacter xylosoxidans and Achromobacter sp. Screening of the degradative ability of the bacteria revealed P. aeruginosa, Bacillus cereus, Acinetobacter calcoaceticus and Achromobacter sp. to be the best degraders. The pH and temperature range with time for the enzyme activity were 6.0-8.0 and 30(o)C-50(o)C respectively. The enzyme exhibited activity that was slightly more tolerant to alkaline pH. Therefore, engineering of Catechol 2,3-dioxygenase may be employed for application on bioremediation of polluted sites.
采用富集技术分离原油降解菌。对分离出的细菌进行降解能力筛选，并根据其生长情况选择最佳降解菌。观察了邻苯二酚-2,3-双加氧酶的比活性、温度和pH的影响及其对酶相对活性的稳定性。从土壤样品中分离出的细菌包括：蜡样芽孢杆菌、解淀粉芽孢杆菌、坚强芽孢杆菌、醋酸钙不动杆菌、假单胞菌属、荧光假单胞菌、恶臭假单胞菌、铜绿假单胞菌、木糖氧化无色杆菌和无色杆菌属。对细菌降解能力的筛选表明铜绿假单胞菌、蜡样芽孢杆菌、醋酸钙不动杆菌和无色杆菌属是最佳降解菌。酶活性随时间变化的pH范围和温度范围分别为6.0-8.0和30(°)C-50(°)C ；该酶表现出对碱性pH稍具耐受性的活性。因此，邻苯二酚2,3-双加氧酶工程可用于污染场地的生物修复。
