Eisenthal Robert, Peterson Michelle E, Daniel Roy M, Danson Michael J
Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, UK.
Trends Biotechnol. 2006 Jul;24(7):289-92. doi: 10.1016/j.tibtech.2006.05.004. Epub 2006 Jun 6.
The way that enzymes respond to temperature is fundamental to many areas of biotechnology. This has long been explained in terms of enzyme stability and catalytic activation energy, but recent observations of enzyme behaviour suggest that this picture is incomplete. We have developed and experimentally validated a new model to describe the effect of temperature on enzymes; this model incorporates additional fundamental parameters that enable a complete description of the effects of temperature on enzyme activity. In this article, we consider the biotechnological implications of this model in the areas of enzyme engineering, enzyme reactor operation and the selection and/or screening of useful enzymes from the environment.
酶对温度的响应方式是生物技术许多领域的基础。长期以来，这一直是根据酶的稳定性和催化活化能来解释的，但最近对酶行为的观察表明，这种描述并不完整。我们已经开发并通过实验验证了一个新模型来描述温度对酶的影响；该模型纳入了额外的基本参数，能够完整描述温度对酶活性的影响。在本文中，我们考虑了该模型在酶工程、酶反应器操作以及从环境中筛选和/或选择有用酶等领域的生物技术意义。
