Papaleo Maria Cristiana, Fondi Marco, Maida Isabel, Perrin Elena, Bevivino Annamaria, Dalmastri Claudia, Fani Renato
Laboratory of Microbial and Molecular Evolution, Department of Biology, University of Florence, Via Madonna del Piano 6, I-50019 Sesto Fiorentino (Florence), Italy.
Technical Unit for Sustainable Development and Innovation of Agro-Industrial System, ENEA Casaccia Research Center, Via Anguillarese 301, 00123 Rome, Italy.
Open Microbiol J. 2015 Aug 31;9:98-109. doi: 10.2174/1874285801509010098. eCollection 2015.
In this work the analysis of the plasmid presence on soil aerobic cultivable heterotrophic bacterial communities was carried out checking a panel of 1,200 isolates, in order to establish the frequency of plasmid presence as well as the degree of plasmid flow between strains affiliated to the same or different taxon. Bacterial communities were isolated from two different sites of a 13-year experimental field with a clay-silt texture. Plasmid molecules were detected at low frequency (27 isolates, 2%) with a size ranging between 2 Kb and 40 Kb. The RAPD analysis performed on the plasmid-harboring isolates and the phylogenetic analysis of the whole community using the 16S rRNA gene sequences revealed the existence of transfer of the same plasmids between strains belonging to the same species and, in some cases, to different species of the same genus. As it might be expected, even though the viable cells title did not differ significantly between the two samplings, the overall data disclosed an uneven distribution of both species and plasmid-harboring strains.
在这项工作中，对土壤需氧可培养异养细菌群落中的质粒存在情况进行了分析，检测了一组1200个分离株，以确定质粒存在的频率以及同一或不同分类群的菌株之间质粒流动的程度。细菌群落是从一个具有粘壤土质地的13年试验田的两个不同地点分离出来的。质粒分子的检测频率较低（27个分离株，2%），大小在2 kb至40 kb之间。对携带质粒的分离株进行的随机扩增多态性DNA（RAPD）分析以及使用16S rRNA基因序列对整个群落进行的系统发育分析表明，同一质粒在同种菌株之间，以及在某些情况下在同属不同种的菌株之间存在转移。正如预期的那样，尽管两次采样之间活细胞数量没有显著差异，但总体数据显示物种和携带质粒的菌株分布不均。
