甲基强的松龙联合胎盘间充质干细胞对兔脊髓损伤模型的神经保护作用
Neuroprotective effect of methylprednisolone combined with placenta-derived mesenchymal stem cell in rabbit model of spinal cord injury.
作者信息
Tan Jiang-Wei, Wang Kai-Yan, Liao Guang-Jun, Chen Fang-Min, Mu Ming-Zhang
机构信息
Department of Spine Surgery, Yantai Affiliated Hospital of Binzhou Medical University Yantai 264100, China.
Department of Anesthesiology, Yantaishan Hospital Yantai 264000, China.
出版信息
Int J Clin Exp Pathol. 2015 Aug 1;8(8):8976-82. eCollection 2015.
The aim of this study was to assess the ability of the combination treatment of methylprednisolone (MP) and placenta-derived mesenchymal stem cells (PDMSCs) in a rabbit model of spinal cord injury (SCI). Rabbits were randomly divided into four groups: group 1 (control), group 2 (MP), group 3 (PDMSCs) and group 4 (MP + PDMSCs). In all groups, the spinal cord injury model was created by the weight drop method. Levels of malondialdehyde (MDA), myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were determined by kit. Histopathological examination was also performed. Neurological evaluation was carried out with the Tarlov scoring system. The results showed both MP and PDMSCs had neuroprotective effects, and combining the administration of MP with PDMSCs was shown a significant effect on the recovery of neurological function. Therefore, the combined use of MP and PDMSCs can be used as a potential therapeutic method for SCI.
本研究旨在评估甲基强的松龙（MP）与胎盘来源间充质干细胞（PDMSCs）联合治疗兔脊髓损伤（SCI）模型的能力。将兔子随机分为四组：第1组（对照组）、第2组（MP组）、第3组（PDMSCs组）和第4组（MP + PDMSCs组）。在所有组中，通过重物坠落法建立脊髓损伤模型。采用试剂盒测定丙二醛（MDA）、髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的水平。还进行了组织病理学检查。采用Tarlov评分系统进行神经学评估。结果表明，MP和PDMSCs均具有神经保护作用，且MP与PDMSCs联合给药对神经功能恢复具有显著效果。因此，MP和PDMSCs联合使用可作为SCI的一种潜在治疗方法。
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