微小RNA-451，一种潜在的胃癌预后生物标志物和肿瘤抑制因子。
MiR-451, a potential prognostic biomarker and tumor suppressor for gastric cancer.
作者信息
Su Zhongxue, Zhao Juan, Rong Zhonghou, Geng Wenmao, Wang Zhiyi
机构信息
Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University 324 Jingwu Road, Jinan 250021, Shandong Province, China.
出版信息
Int J Clin Exp Pathol. 2015 Aug 1;8(8):9154-60. eCollection 2015.
BACKGROUND
The expression of microRNA-451 (miR-451) and its association with clinical pathological factors in GC remain still unclear. The purpose of this study was to investigate if miR-451 is a potential prognostic biomarker and tumor suppressor for gastric cancer.
METHODS
Real-time quantitative RT-PCR (qRT-PCR) was applied to detect miR-451 expression in GC cell lines and primary tumor and paired non-cancerous tissues. The association of miR-451 expression with clinicopathological factors and prognosis was statistically analyzed.
RESULTS
We found that miR-451 showed decreased expression in GC tissues and cell lines, and its down-regulation tended to be positively correlated with lymphatic metastasis, clinical staging and shorter overall survival of patients. In addition, forced expression of miR-451 in BGC-823 and MKN-45 cells did not impact on cell proliferation, but did reduce migration and invasion rates in BGC-823 cells.
CONCLUSION
Our findings indicated that miR-451 may act as a novel prognostic marker and potential therapeutic target in human GC.
背景
微小RNA-451（miR-451）在胃癌（GC）中的表达及其与临床病理因素的关系仍不清楚。本研究旨在探讨miR-451是否为胃癌潜在的预后生物标志物和肿瘤抑制因子。
方法
应用实时定量逆转录聚合酶链反应（qRT-PCR）检测miR-451在GC细胞系、原发性肿瘤及配对的癌旁组织中的表达。对miR-451表达与临床病理因素及预后的相关性进行统计学分析。
结果
我们发现miR-451在GC组织和细胞系中表达降低，其下调与患者的淋巴转移、临床分期及较短的总生存期呈正相关。此外，在BGC-823和MKN-45细胞中强制表达miR-451对细胞增殖无影响，但可降低BGC-823细胞的迁移和侵袭率。
结论
我们的研究结果表明，miR-451可能是人类GC的一种新型预后标志物和潜在的治疗靶点。
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