Su Zhongxue, Zhao Juan, Rong Zhonghou, Geng Wenmao, Wang Zhiyi
Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University 324 Jingwu Road, Jinan 250021, Shandong Province, China.
Int J Clin Exp Pathol. 2015 Aug 1;8(8):9154-60. eCollection 2015.
The expression of microRNA-451 (miR-451) and its association with clinical pathological factors in GC remain still unclear. The purpose of this study was to investigate if miR-451 is a potential prognostic biomarker and tumor suppressor for gastric cancer.
Real-time quantitative RT-PCR (qRT-PCR) was applied to detect miR-451 expression in GC cell lines and primary tumor and paired non-cancerous tissues. The association of miR-451 expression with clinicopathological factors and prognosis was statistically analyzed.
We found that miR-451 showed decreased expression in GC tissues and cell lines, and its down-regulation tended to be positively correlated with lymphatic metastasis, clinical staging and shorter overall survival of patients. In addition, forced expression of miR-451 in BGC-823 and MKN-45 cells did not impact on cell proliferation, but did reduce migration and invasion rates in BGC-823 cells.
Our findings indicated that miR-451 may act as a novel prognostic marker and potential therapeutic target in human GC.
微小RNA-451（miR-451）在胃癌（GC）中的表达及其与临床病理因素的关系仍不清楚。本研究旨在探讨miR-451是否为胃癌潜在的预后生物标志物和肿瘤抑制因子。
应用实时定量逆转录聚合酶链反应（qRT-PCR）检测miR-451在GC细胞系、原发性肿瘤及配对的癌旁组织中的表达。对miR-451表达与临床病理因素及预后的相关性进行统计学分析。
我们发现miR-451在GC组织和细胞系中表达降低，其下调与患者的淋巴转移、临床分期及较短的总生存期呈正相关。此外，在BGC-823和MKN-45细胞中强制表达miR-451对细胞增殖无影响，但可降低BGC-823细胞的迁移和侵袭率。
我们的研究结果表明，miR-451可能是人类GC的一种新型预后标志物和潜在的治疗靶点。
