Li Chunjing, Shu Fangpeng, Lei Bin, Lv Daojun, Zhang Shoubo, Mao Xiangming
Department of Urology, Nanfang Hospital, Southern Medical University Guangzhou, Guangdong Province, China ; Department of Surgery, Women and Children's Hospital of Foshan Foshan, Guangdong, Province, China.
Department of Urology, Nanfang Hospital, Southern Medical University Guangzhou, Guangdong Province, China.
Int J Clin Exp Pathol. 2015 Aug 1;8(8):9410-5. eCollection 2015.
This study is aimed to evaluate the expression of phosphoglycerate mutase 1 (PGAM1) in normal kidney and clear cell renal cell carcinoma (CCRCC), also to evaluate the correlation between PGAM1 expression and clinicopathological features in CCRCC.
PGAM1 expression was detected in 80 cases of normal kidney and 192 cases of CCRCC by immunohistochemistry (IHC). Meanwhile, PGAM1 expression measured in 8 cases of CCRCC and matched normal kidney tissues by Western blot. Then, the correlation between PGAM1 expression and clinicalpathological features was analyzed in CCRCC.
IHC results exhibited that the high-expression rate of PGAM1 in CCRCC tissues was 45.8%, which was significantly higher than those in normal kidney tissues (32.5%, P=0.044). Meanwhile, PGAM1 expression in CCRCC was significantly greater compared with those in normal kidney by Western blot. Moreover, PGAM1 expression was significantly associated with age, tumor size and T stage in CCRCC.
PGAM1 is highly expressed in CCRCC and correlated with clinicalpathological features, which may contribute to tumor formation and progression.
本研究旨在评估磷酸甘油酸变位酶1（PGAM1）在正常肾组织和透明细胞肾细胞癌（CCRCC）中的表达情况，并评估PGAM1表达与CCRCC临床病理特征之间的相关性。
采用免疫组织化学（IHC）方法检测80例正常肾组织和192例CCRCC组织中PGAM1的表达。同时，采用蛋白质免疫印迹法检测8例CCRCC组织及其配对的正常肾组织中PGAM1的表达。然后，分析CCRCC中PGAM1表达与临床病理特征之间的相关性。
免疫组织化学结果显示，CCRCC组织中PGAM1的高表达率为45.8%，显著高于正常肾组织（32.5%，P = 0.044）。同时，蛋白质免疫印迹法显示CCRCC中PGAM1的表达明显高于正常肾组织。此外，PGAM1表达与CCRCC患者的年龄、肿瘤大小及T分期显著相关。
PGAM1在CCRCC中高表达，且与临床病理特征相关，这可能促进肿瘤的形成和进展。
