Guo Changcheng, Xiong Dabo, Yao Xudong, Gu Wenyu, Zhang Haimin, Yang Bin, Peng Bo, Liu Min, Zheng Junhua
Department of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 200072, People's Republic of China.
Int J Clin Exp Pathol. 2015 Apr 1;8(4):3710-8. eCollection 2015.
In this study, we investigate the expression and role of special AT-rich sequence-binding protein-2 (SATB2) in clear cell renal cell carcinoma (ccRCC) tissue, and to evaluate the clinical and prognostic significance of SATB2 protein in patients with ccRCC. The expression of SATB2 and SATB1 was examined in ccRCC tissue by Western blotting, real-time PCR and immunohistochemical staining. The association between clinicopathological features and SATB2 level was investigated. The correlation of SATB2 expression with overall survival was also analyzed. The expression of SATB2 protein in tumor tissues was much lower than that in paired normal tissues. The overall survival of the patients with high SATB2 expression was significantly higher than that of the low SATB2 expression group. Low or negative SATB2 expression was significantly correlated with AJCC staging and Furman grade in ccRCC. In contrast, the expression of SATB1 was significantly higher in adjacent tumor tissue than that in normal and tumor tissues. This study provides the first evidence of the expression and clinical significance of SATB2 in ccRCC. Our data suggest that SATB2 functions as a tumor suppressor in the development and progression of ccRCC, and is thereby implicated as a valuable prognostic marker for ccRCC patients.
在本研究中，我们调查了富含AT序列结合蛋白2（SATB2）在透明细胞肾细胞癌（ccRCC）组织中的表达及作用，并评估SATB2蛋白在ccRCC患者中的临床和预后意义。通过蛋白质免疫印迹法、实时荧光定量PCR和免疫组织化学染色检测ccRCC组织中SATB2和SATB1的表达。研究临床病理特征与SATB2水平之间的关联。还分析了SATB2表达与总生存期的相关性。肿瘤组织中SATB2蛋白的表达远低于配对的正常组织。SATB2高表达患者的总生存期显著高于SATB2低表达组。SATB2低表达或阴性表达与ccRCC中的美国癌症联合委员会（AJCC）分期和富尔曼分级显著相关。相反，SATB1在肿瘤旁组织中的表达显著高于正常组织和肿瘤组织。本研究首次提供了SATB2在ccRCC中表达及临床意义的证据。我们的数据表明，SATB2在ccRCC的发生和发展中起肿瘤抑制作用，因此被认为是ccRCC患者有价值的预后标志物。
