Department of Neurology, Massachusetts General Hospital , Boston, Massachusetts 02129 ; Seoul Paik Hospital, Inje University , Seoul 100-032, South Korea.
Department of Neurology, Massachusetts General Hospital , Boston, Massachusetts 02129 ; Harvard Medical School , Boston, Massachusetts 02129.
eNeuro. 2015 Mar 25;2(2). doi: 10.1523/ENEURO.0027-14.2015. eCollection 2015 Mar-Apr.
Epileptogenesis in vivo can be altered by manipulation of molecules such as cytokines and complement that subserve intercellular signaling in both the inflammatory and central nervous systems. Because of the dual roles of these signaling molecules, it has been difficult to precisely define the role of systemic inflammation in epileptogenesis. Organotypic hippocampal brain slices can be maintained in culture independently of the systemic inflammatory system, and the rapid course of epileptogenesis in these cultures supports the idea that inflammation is not necessary for epilepsy. However, this preparation still retains key cellular inflammatory mediators. Here, we found that rodent hippocampal organotypic slice cultures depleted of T lymphocytes and microglia developed epileptic activity at essentially the same rate and to similar degrees of severity as matched control slice cultures. These data support the idea that although the inflammatory system, neurons, and glia share key intercellular signaling molecules, neither systemic nor CNS-specific cellular elements of the immune and inflammatory systems are necessary components of epileptogenesis.
在体内，通过操纵细胞因子和补体等分子，可以改变癫痫发生，这些分子在炎症和中枢神经系统中都具有细胞间信号转导作用。由于这些信号分子的双重作用，很难准确确定全身炎症在癫痫发生中的作用。器官型海马脑片可以在独立于全身炎症系统的情况下进行培养，并且这些培养物中癫痫发生的快速过程支持炎症不是癫痫发生所必需的观点。然而，这种制备方法仍然保留了关键的细胞炎症介质。在这里，我们发现，耗尽 T 淋巴细胞和小胶质细胞的啮齿动物海马器官型脑片培养物以与匹配的对照脑片培养物基本相同的速度和相似的严重程度发展出癫痫活动。这些数据支持这样一种观点，即尽管炎症系统、神经元和神经胶质细胞共享关键的细胞间信号分子，但免疫和炎症系统的全身或中枢神经系统特异性细胞成分都不是癫痫发生的必要组成部分。
