Carvalho Maria de Fátima Pereira de, Pereira Celina Siqueira Barbosa, Fregnani José Humberto, Ribeiro Fernando de Andrade Quintanilha
Department of Morphology, Santa Casa School of Medical Sciences of Sao Paulo, Universidade de Sao Paulo, BR.
Department of Otorhinolaryngology, Santa Casa School of Medical Sciences of Sao Paulo, USP, Sao Paulo, SP, BR.
Acta Cir Bras. 2015 Sep;30(9):593-7. doi: 10.1590/S0102-865020150090000002.
To compare histologically the action of Mitomycin C and that of Clobetasol propionate for surgical wound healing in rats.
A circular skin fragment was surgically removed from 57 Wistar rats. Twenty-two animals were treated with Mitomycin C with topical medication in a single dose, 22 with Clobetasol propionate with a cream medication once a day for 15 days and 13 did not receive any medication. The animals were euthanized 30 and 60 days, and the scars subjected to histological examination.
The histological analysis on the samples did not show statistically significant differences regarding the quantities of fibroblasts, fibrocytes and vascular proliferation in the three groups, in the evaluations after 30 and 60 days. In the treated groups with Mitomycin C and Clobetasol there was a decrease in collagen concentration over the 30-day period and an increase in collagen concentration over the 60-day period, in comparison with the control group.
The actions of Mitomycin C and Clobetasol were equivalent and not interfere in fibroplasias and in angiogenesis. Both drugs initially cause a decrease in collagen over a 30-day period and an increase over a 60-day period, demonstrating a delay in the wound healing.
通过组织学方法比较丝裂霉素C和丙酸氯倍他索对大鼠手术伤口愈合的作用。
从57只Wistar大鼠身上手术切除圆形皮肤碎片。22只动物单次局部应用丝裂霉素C治疗，22只动物每天一次外用丙酸氯倍他索乳膏，持续15天，13只动物未接受任何药物治疗。在30天和60天时对动物实施安乐死，并对瘢痕进行组织学检查。
在30天和60天的评估中，对样本的组织学分析显示，三组中成纤维细胞、纤维细胞数量及血管增生情况无统计学显著差异。与对照组相比，丝裂霉素C和丙酸氯倍他索治疗组在30天内胶原浓度降低，在60天内胶原浓度升高。
丝裂霉素C和丙酸氯倍他索的作用相当，且不影响纤维形成和血管生成。两种药物最初在30天内导致胶原减少，在60天内导致胶原增加，表明伤口愈合延迟。
