Strachecka Aneta, Paleolog Jerzy, Olszewski Krzysztof, Borsuk Grzegorz
Laboratory of Experimental and Environmental Biology, Department of Biological Basis of Animal Production, University of Life Sciences in Lublin, ul. Akademicka 13, PL-20-950 Lublin, Poland.
Insects. 2012 Aug 27;3(3):821-32. doi: 10.3390/insects3030821.
This work verifies that amitraz and oxalic acid treatment affect honeybee cuticle proteolytic enzymes (CPE). Three bee groups were monitored: oxalic acid treatment, amitraz treatment, control. Electrophoresis of hydrophilic and hydrophobic CPE was performed. Protease and protease inhibitor activities (in vitro) and antifungal/antibacterial efficiencies (in vivo), were analyzed. Amitraz and oxalic acid treatment reduced hydrophobic, but did not affect hydrophilic, protein concentrations and reduced both hydrophilic and hydrophobic body surface asparagine and serine protease activities in relation to most substrates and independently of pH. The activities of natural cuticle inhibitors of acidic, neutral, and alkaline proteases were suppressed as a result of the treatments, corresponding with reduced antifungal and antibacterial activity. Electrophoretic patterns of low-, medium-, and high-molecular-weight proteases and protease inhibitors were also affected by the treatments.
这项研究证实，双甲脒和草酸处理会影响蜜蜂表皮蛋白水解酶（CPE）。监测了三组蜜蜂：草酸处理组、双甲脒处理组、对照组。对亲水性和疏水性CPE进行了电泳分析。分析了蛋白酶和蛋白酶抑制剂活性（体外）以及抗真菌/抗菌效率（体内）。双甲脒和草酸处理降低了疏水性，但不影响亲水性蛋白质浓度，并且相对于大多数底物且不受pH影响，降低了亲水性和疏水性体表天冬酰胺和丝氨酸蛋白酶的活性。由于处理，酸性、中性和碱性蛋白酶的天然表皮抑制剂的活性受到抑制，这与抗真菌和抗菌活性降低相对应。低、中、高分子量蛋白酶和蛋白酶抑制剂的电泳图谱也受到处理的影响。
