Blanchard Philippe, Schurr Frank, Celle Olivier, Cougoule Nicolas, Drajnudel Patrick, Thiéry Richard, Faucon Jean-Paul, Ribière Magali
Agence Française de Sécurité Sanitaire des Aliments Sophia-Antipolis, Unité Pathologie de I'Abeille, Les Templiers, Route des Chappes, BP 111, 06902 Sophia Antipolis, France.
J Invertebr Pathol. 2008 Nov;99(3):348-50. doi: 10.1016/j.jip.2008.07.006. Epub 2008 Jul 30.
Bee samples were collected in French apiaries that displayed severe losses and mortality during the winter (from November 2007 to March 2008). They were screened for the presence of Israeli acute paralysis virus (IAPV) by using RT-PCR. Five out of 35 surveyed apiaries, located in two different geographical areas, were found positive. This represents the first reported detection of IAPV in France. The specificity of the PCR products was checked by sequencing. The phylogenetic analysis showed that French isolates of IAPV were closely related to a cluster including American and Australian isolates. Nevertheless, most of American isolates previously reported to be associated to Colony Collapse Disorder (CCD) and an Israeli isolate first isolated in 2004 from dead bees were included in another cluster. Since IAPV was detected in only 14% of the affected apiaries, it was not possible to establish a causal link between IAPV and the severe winter losses that occurred.
在法国一些冬季（2007年11月至2008年3月）出现严重损失和死亡情况的养蜂场采集了蜜蜂样本。通过逆转录聚合酶链反应（RT-PCR）检测样本中是否存在以色列急性麻痹病毒（IAPV）。在位于两个不同地理区域的35个接受调查的养蜂场中，有5个被检测出呈阳性。这是法国首次报告检测到IAPV。通过测序检查了PCR产物的特异性。系统发育分析表明，法国的IAPV分离株与包括美国和澳大利亚分离株在内的一个簇密切相关。然而，之前报道的大多数与蜂群崩溃失调（CCD）相关的美国分离株以及2004年首次从死蜂中分离出的一株以色列分离株被归入另一个簇。由于仅在14%的受影响养蜂场中检测到IAPV，因此无法确定IAPV与所发生的严重冬季损失之间的因果关系。
