Oberle Jennifer, Dighton John, Arbuckle-Keil Georgia
Rutgers University, School of Environmental and Biological Sciences, Department of Ecology and Evolution, 14 College Farm Road, New Brunswick, NJ 08901-8551, USA.
Rutgers University, Rutgers Pinelands Field Station, PO Box 206, 501 Four Mile Road, New Lisbon, NJ 08064, USA.
Fungal Biol. 2015 Nov;119(11):1100-1114. doi: 10.1016/j.funbio.2015.08.007. Epub 2015 Aug 24.
Twenty distinct fungal isolates were analysed using three methods of sample preparation for FTIR spectroscopy and FTIR-ATR microspectroscopy to test for differences in surface chemical composition between living and dried fungal samples, as well as differences between surface chemistry and overall chemistry of homogenized dried samples. Results indicated that visually the FTIR spectra of different fungi are remarkably similar with subtle discernable differences, which statistical analysis of the spectra supported. Within each data set, different fungal isolates were responsible for statistical differences. Lack of congruence between each of the methods used suggests that determination of chemical composition is highly dependent upon the method of sample preparation and analysis (surface vs. whole) applied.
使用三种样品制备方法对20种不同的真菌分离株进行傅里叶变换红外光谱（FTIR）和傅里叶变换红外衰减全反射（FTIR-ATR）显微光谱分析，以测试活真菌样品和干燥真菌样品之间表面化学成分的差异，以及均质干燥样品的表面化学与整体化学之间的差异。结果表明，从视觉上看，不同真菌的FTIR光谱非常相似，只有细微的可辨别差异，光谱的统计分析也支持这一点。在每个数据集中，不同的真菌分离株导致了统计差异。所使用的每种方法之间缺乏一致性，这表明化学成分的测定高度依赖于所应用的样品制备和分析方法（表面分析与整体分析）。
