Hosnuter Mubin, Melikoglu Cenk, Aslan Cem, Saglam Gulcan, Sutcu Recep
Department of Plastic, Reconstructive and Aesthetic Surgery, Katip Celebi University School of Medicine, Ataturk Training and Research Hospital, Izmir, Turkey.
Department of Plastic, Reconstructive and Aesthetic Surgery, Sifa University School of Medicine, Izmir, Turkey.
Adv Clin Exp Med. 2015 May-Jun;24(3):409-17. doi: 10.17219/acem/43761.
Epigallocatechin gallate (EGCG), a green tea polyphenol, has potent antioxidant properties.
The purpose of the present study was to examine the possible preventative effects of EGCG against internal organ injury due to large-surface skin burns in a rat model.
The study design involved three groups of rats: a sham group and two groups with 25-30% full-thickness burns: (a) the sham group without burns or treatment (n=18); (b) the control burn group (burns+sterile saline, n=18); and (c) the burn treatment group (burns+treatment with EGCG, n=18). EGCG was administered intraperitoneally immediately after the thermal injury, and daily in 100 μmol/kg doses. Kidney and lung tissue samples were taken to determine the levels of malondialdehyde (MDA), superoxide dismutase (SOD), tumor necrosis factor-α (TNF-α), and glutathione peroxidase (GPX) after the first, third and seventh post-burn days.
In the EGCG-treated burn group, SOD and GPX activity were significantly higher than in the burn control group. Additionally, MDA and TNF-α levels were significantly lower in the EGCG-treated burn group.
Based on this study, it might be anticipated that EGCG treatment may be beneficial in burn injury cases.
表没食子儿茶素没食子酸酯（EGCG）是一种绿茶多酚，具有强大的抗氧化特性。
本研究的目的是在大鼠模型中研究EGCG对大面积皮肤烧伤所致内脏器官损伤的可能预防作用。
研究设计包括三组大鼠：假手术组和两组25 - 30%全层烧伤组：（a）未烧伤或未治疗的假手术组（n = 18）；（b）对照烧伤组（烧伤+无菌生理盐水，n = 18）；（c）烧伤治疗组（烧伤+EGCG治疗，n = 18）。热损伤后立即腹腔注射EGCG，每日剂量为100 μmol/kg。在烧伤后第1、3和7天采集肾脏和肺组织样本，以测定丙二醛（MDA）、超氧化物歧化酶（SOD）、肿瘤坏死因子-α（TNF-α）和谷胱甘肽过氧化物酶（GPX）的水平。
在EGCG治疗的烧伤组中，SOD和GPX活性显著高于烧伤对照组。此外，EGCG治疗的烧伤组中MDA和TNF-α水平显著降低。
基于本研究，预计EGCG治疗可能对烧伤病例有益。
