非组蛋白染色体蛋白对WI-38人二倍体成纤维细胞中组蛋白基因转录的激活作用。
Activation of histone gene transcription by nonhistone chromosomal proteins in WI-38 human diploid fibroblasts.
作者信息
Jansing R L, Stein J L, Stein G S
出版信息
Proc Natl Acad Sci U S A. 1977 Jan;74(1):173-7. doi: 10.1073/pnas.74.1.173.
Abstract
The regulation of histone gene expression was examined after confluent, nondividing WI-38 human diploid fibroblasts were stimulated to proliferate. Histone mRNA sequences were assayed by hybrid formation with an 3H-labeled single-stranded DNA complementary to histone mRNAs. Histone mRNA became associated with polyribosomes concomitant with the activation of DNA synthesis. The ability of chromatin from WI-38 cells to serve as a template for in vitro transcription of histone mRNA sequences parallels the onset of DNA replication. A role for nonhistone chromosomal proteins in the control of histone gene readout is suggested because, when chromatin from confluent WI-38 cells was dissociated and then reconstituted in the presence of S phase nonhistone chromosomal proteins, a 500-fold activation of histone mRNA sequence transcription was observed.
摘要
在汇合的、不分裂的WI-38人二倍体成纤维细胞被刺激增殖后，对组蛋白基因表达的调控进行了研究。通过与与组蛋白mRNA互补的3H标记单链DNA形成杂交来测定组蛋白mRNA序列。组蛋白mRNA与多核糖体的结合伴随着DNA合成的激活。WI-38细胞的染色质作为组蛋白mRNA序列体外转录模板的能力与DNA复制的开始平行。非组蛋白染色体蛋白在组蛋白基因读出控制中的作用被提出，因为当汇合的WI-38细胞的染色质被解离，然后在S期非组蛋白染色体蛋白存在下重新组装时，观察到组蛋白mRNA序列转录有500倍的激活。
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本文引用的文献
1
The Biology of Isolated Chromatin: Chromosomes, biologically active in the test tube, provide a powerful tool for the study of gene action.孤立染色质的生物学：在试管中具有生物活性的染色体为研究基因作用提供了一个有力的工具。
Science. 1968 Jan 5;159(3810):47-56. doi: 10.1126/science.159.3810.47.
2
The cytoplasmic synthesis of histones in hela cells and its temporal relationship to DNA replication.海拉细胞中组蛋白的细胞质合成及其与DNA复制的时间关系。
Proc Natl Acad Sci U S A. 1967 Feb;57(2):409-16. doi: 10.1073/pnas.57.2.409.
3
Role of non-histone components in determining organ specificity of rabbit chromatins.非组蛋白成分在决定兔染色质器官特异性中的作用。
FEBS Lett. 1970 Aug 17;9(4):242-244. doi: 10.1016/0014-5793(70)80366-0.
4
Organ-specific restriction of transcription in mammalian chromatin.哺乳动物染色质中转录的器官特异性限制。
J Mol Biol. 1968 Jul 14;34(2):305-16. doi: 10.1016/0022-2836(68)90255-6.
5
Control of nucleic acid synthesis in human diploid cells undergoing contact inhibition.处于接触抑制状态的人二倍体细胞中核酸合成的调控。
Exp Cell Res. 1968 Oct;53(1):184-204. doi: 10.1016/0014-4827(68)90366-2.
6
Deoxyribonucleoproteins and the tissue-specific restriction of the deoxyribonucleic acid in chromatin.脱氧核糖核蛋白与染色质中脱氧核糖核酸的组织特异性限制
Biochem J. 1970 Nov;120(2):435-7. doi: 10.1042/bj1200435.
7
Specific binding of rat liver nuclear proteins to DNA.大鼠肝脏核蛋白与DNA的特异性结合。
Nature. 1970 Jun 13;226(5250):1025-6. doi: 10.1038/2261025a0.
8
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10
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Proc Natl Acad Sci U S A. 1967 Nov;58(5):1977-83. doi: 10.1073/pnas.58.5.1977.
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