组蛋白和非组蛋白染色体蛋白对HeLaS3细胞DNA中组蛋白基因转录的影响。

Effect of histones and nonhistone chromosomal proteins on the transcription of histone genes from HeLaS3 cell DNA.

作者信息

Stein J L, Reed K, Stein G S

出版信息

Biochemistry. 1976 Jul 27;15(15):3291-5. doi: 10.1021/bi00660a019.

Abstract

To elucidate the manner in which histones and nonhistone chromosomal proteins interact to render histone genes transcribable in HeLa S3 cells, we have examined transcription of histone mRNA sequences from DNA, as well as from several DNA-chromosomal protein complexes. Histone mRNA sequences were assayed by hybridization to a 3H-labeled single-stranded DNA complementary to histone mRNAs. Our results indicate that DNA is an effective template for transcription of histone mRNA sequences and that histones by themselves inhibit transcription from DNA, including transcription of histone genes, in a dose-dependent, nonspecific manner. When complexed with DNA alone, nonhistone chromosomal proteins do not affect the transcription of histone mRNA sequences. However, when associated with DNA in the presence of histones, nonhistone chromosomal proteins are capable of selectively rendering histone genes transcribable. These results suggest a possible role for nonhistone chromosomal proteins in mediating the interactions of histones with DNA to render histone genes transcribable.

摘要

为阐明组蛋白与非组蛋白染色体蛋白相互作用以使组蛋白基因在HeLa S3细胞中可转录的方式，我们检测了来自DNA以及几种DNA-染色体蛋白复合物的组蛋白mRNA序列的转录情况。通过与与组蛋白mRNA互补的3H标记单链DNA杂交来检测组蛋白mRNA序列。我们的结果表明，DNA是组蛋白mRNA序列转录的有效模板，并且组蛋白自身以剂量依赖性、非特异性方式抑制来自DNA的转录，包括组蛋白基因的转录。当仅与DNA复合时，非组蛋白染色体蛋白不影响组蛋白mRNA序列的转录。然而，当在组蛋白存在下与DNA结合时，非组蛋白染色体蛋白能够选择性地使组蛋白基因可转录。这些结果表明非组蛋白染色体蛋白在介导组蛋白与DNA的相互作用以使组蛋白基因可转录方面可能发挥作用。