Geng Fang, Huang Xi, Ma Meihu
National R&D Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China.
J Sci Food Agric. 2016 Jul;96(9):3188-94. doi: 10.1002/jsfa.7498. Epub 2015 Nov 18.
Hen egg white ovomacroglobulin (OVM) possesses a variety of bioactivities and could potentially be used as a pharmaceutical agent. It has been reported that OVM is involved in wound healing and cancer pathological processes, and previous results suggest that OVM plays a potential role in cell proliferation and migration; however, this has not yet been proven. In the present study, the effects of OVM on fibroblast proliferation and migration were evaluated.
Results of cell counting, cell viability, and cell cycle indicated that proliferation of fibroblasts was not altered by OVM treatment. However, scratch assays showed that OVM could promote the migration of 3 T6 mouse embryonic fibroblasts and human skin fibroblasts (HSF). Also, the adhesion of HSF to the collagen matrix was also enhanced by OVM treatment. RT-qPCR and western blot analysis showed that β1 -integrin, β-tubulin, and β-actin were up-regulated while E-cadherin was down-regulated in OVM-treated HSF cells. The effect of OVM was silenced after forming a complex with trypsin, suggesting that the protease inhibitory ability of OVM is important for its effect on cell migration.
These results suggested that promotion of OVM on cell migration was achieved by enhancing cell adhesion to extracellular matrix, reducing intercellular aggregation, and strengthening cytoskeleton. The finding of the promotion effect of OVM on cell migration is important for understanding its role in wound healing and cancer pathological processes. © 2015 Society of Chemical Industry.
鸡卵清白蛋白（OVM）具有多种生物活性，有可能用作药物制剂。据报道，OVM参与伤口愈合和癌症病理过程，先前的结果表明OVM在细胞增殖和迁移中发挥潜在作用；然而，这尚未得到证实。在本研究中，评估了OVM对成纤维细胞增殖和迁移的影响。
细胞计数、细胞活力和细胞周期结果表明，OVM处理未改变成纤维细胞的增殖。然而，划痕试验表明，OVM可促进3T6小鼠胚胎成纤维细胞和人皮肤成纤维细胞（HSF）的迁移。此外，OVM处理还增强了HSF与胶原基质的黏附。RT-qPCR和蛋白质印迹分析表明，在OVM处理的HSF细胞中，β1整合素、β微管蛋白和β肌动蛋白上调，而E-钙黏蛋白下调。与胰蛋白酶形成复合物后，OVM的作用被消除，这表明OVM的蛋白酶抑制能力对其细胞迁移作用很重要。
这些结果表明，OVM对细胞迁移的促进作用是通过增强细胞与细胞外基质的黏附、减少细胞间聚集和强化细胞骨架实现的。OVM对细胞迁移促进作用的发现对于理解其在伤口愈合和癌症病理过程中的作用很重要。©2015化学工业协会。
