Department of Biology of Animal Environment, Faculty of Animal Science, Warsaw University of Life Sciences, Ciszewskiego street 8, 02-786 Warsaw, Poland.
Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego street 8, 02-786 Warsaw, Poland.
Toxicol In Vitro. 2018 Apr;48:276-285. doi: 10.1016/j.tiv.2018.01.028. Epub 2018 Feb 1.
The aim of the present study was to evaluate the cytotoxicity of pristine graphene monolayer and its utility as a scaffold for murine fibroblast L929 cell line. Cell viability, morphology, cytoskeleton architecture (microfilaments and microtubules), cell adhesion and migration into the scratch-wound area were determined using pristine graphene-coated microscopic slides. We found that fibroblasts cultured on pristine graphene monolayer exhibited changes in cell attachment, motility and cytoskeleton organization. Graphene was found to have no cytotoxicity on L929 fibroblasts and increased cell adhesion and proliferation within 24 h of culture. The area of cells growing on graphene was comparable to the area of fibroblasts cultured on glass. Migration of cells on the surface of graphene substrate appeared to be more regular in comparison to uncoated glass surface, however in both control (glass) and experimental (graphene) groups the scratch wound was closed after 48 h of culture. Taken together, our results indicate that pristine graphene monolayer is non-toxic for murine subcutaneous connective tissue fibroblasts and could be beneficial for recovery of damaged tissues after injury. These studies could be helpful in evaluating biocompatibility of graphene, which still remains ambiguous.
本研究旨在评估原始石墨烯单层的细胞毒性及其作为鼠成纤维细胞 L929 系支架的用途。使用原始石墨烯涂覆的载玻片测定细胞活力、形态、细胞骨架结构（微丝和微管）、细胞黏附和向划痕区域的迁移。我们发现，在原始石墨烯单层上培养的成纤维细胞表现出细胞附着、运动和细胞骨架组织的变化。在培养 24 小时内，发现石墨烯对 L929 成纤维细胞没有细胞毒性，并且增加了细胞黏附和增殖。在石墨烯上生长的细胞面积与在玻璃上培养的成纤维细胞面积相当。与未涂覆的玻璃表面相比，石墨烯基底表面上细胞的迁移似乎更加规则，但是在对照（玻璃）和实验组（石墨烯）中，培养 48 小时后划痕伤口均已闭合。总之，我们的结果表明，原始石墨烯单层对鼠皮下结缔组织成纤维细胞无毒，并且可能有益于受伤后受损组织的恢复。这些研究有助于评估石墨烯的生物相容性，目前其生物相容性仍不明确。
