Harrington J P, Elbaum D, Bookchin R M, Wittenberg J B, Nagel R L
Proc Natl Acad Sci U S A. 1977 Jan;74(1):203-6. doi: 10.1073/pnas.74.1.203.
Oxygen uptake of fully deoxygenated sickle (SS) erythrocytes is slower than that of normal (AA) erythrocytes, as demonstrated by the half-times of the overall oxygenation reactions: at 25 degrees in an isotonic phosphate buffer the normal red cells have a t1/2 = 82 +/- 4.7 msec, as compared to sickle red cells where t1/2 = 135 +/- 17.6 msec. The effects of temperature, extracellular osmolality, and the presence of an antisickling agent (n-butylurea) on the rate of red cell oxygenation strongly suggest that the differences in oxygenation rates encountered with sickle red cells is directly related to the intracellular polymerization of deoxyhemoglobin S.
完全脱氧的镰状(SS)红细胞的摄氧速度比正常(AA)红细胞慢,这一点可通过整体氧合反应的半衰期来证明:在25摄氏度的等渗磷酸盐缓冲液中,正常红细胞的t1/2 = 82±4.7毫秒,而镰状红细胞的t1/2 = 135±17.6毫秒。温度、细胞外渗透压以及抗镰变剂(正丁基脲)的存在对红细胞氧合速率的影响有力地表明,镰状红细胞氧合速率的差异与脱氧血红蛋白S在细胞内的聚合直接相关。