含血红蛋白S的红细胞的配体动力学
Ligand kinetics of hemoglobin S containing erythrocytes.
作者信息
Harrington J P, Elbaum D, Bookchin R M, Wittenberg J B, Nagel R L
出版信息
Proc Natl Acad Sci U S A. 1977 Jan;74(1):203-6. doi: 10.1073/pnas.74.1.203.
Abstract
Oxygen uptake of fully deoxygenated sickle (SS) erythrocytes is slower than that of normal (AA) erythrocytes, as demonstrated by the half-times of the overall oxygenation reactions: at 25 degrees in an isotonic phosphate buffer the normal red cells have a t1/2 = 82 +/- 4.7 msec, as compared to sickle red cells where t1/2 = 135 +/- 17.6 msec. The effects of temperature, extracellular osmolality, and the presence of an antisickling agent (n-butylurea) on the rate of red cell oxygenation strongly suggest that the differences in oxygenation rates encountered with sickle red cells is directly related to the intracellular polymerization of deoxyhemoglobin S.
摘要
完全脱氧的镰状（SS）红细胞的摄氧速度比正常（AA）红细胞慢，这一点可通过整体氧合反应的半衰期来证明：在25摄氏度的等渗磷酸盐缓冲液中，正常红细胞的t1/2 = 82±4.7毫秒，而镰状红细胞的t1/2 = 135±17.6毫秒。温度、细胞外渗透压以及抗镰变剂（正丁基脲）的存在对红细胞氧合速率的影响有力地表明，镰状红细胞氧合速率的差异与脱氧血红蛋白S在细胞内的聚合直接相关。
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