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一种新型葡萄球菌盒式染色体mec XI型引物，用于直接从筛查标本中检测携带mecC的耐甲氧西林金黄色葡萄球菌。

A Novel Staphylococcal Cassette Chromosome mec Type XI Primer for Detection of mecC-Harboring Methicillin-Resistant Staphylococcus aureus Directly from Screening Specimens.

作者信息

Petersdorf Sabine, Herma Miriam, Rosenblatt Meike, Layer Franziska, Henrich Birgit

机构信息

Institute of Medical Microbiology and Hospital Hygiene, University Clinic of Heinrich-Heine University Düsseldorf, Düsseldorf, Germany.

Robert Koch Institute, Department of Infectious Diseases, National Centre for Staphylococci and Enterococci, Wernigerode, Gemany.

出版信息

J Clin Microbiol. 2015 Dec;53(12):3938-41. doi: 10.1128/JCM.02328-15. Epub 2015 Oct 14.

DOI:10.1128/JCM.02328-15
PMID:26468503
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4652094/
Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) screening using real-time PCR has decreased in sensitivity due to the emergence of variant staphylococcal cassette chromosome mec element (SCCmec) types. We have designed and validated a novel SCCmec XI primer, which enables for the first time the rapid detection of mecC-harboring MRSA directly from nasopharyngeal swabs without prior cultivation.

摘要

由于新型葡萄球菌盒式染色体mec元件（SCCmec）类型的出现，使用实时聚合酶链反应（PCR）进行耐甲氧西林金黄色葡萄球菌（MRSA）筛查的灵敏度有所下降。我们设计并验证了一种新型的SCCmec XI引物，首次实现了直接从鼻咽拭子中快速检测携带mecC的MRSA，无需事先培养。

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