临床实验室检测耐碳青霉烯类肠杆菌科细菌面临的持续挑战。
Continuing Challenges for the Clinical Laboratory for Detection of Carbapenem-Resistant Enterobacteriaceae.
作者信息
Humphries Romney M, McKinnell James A
机构信息
Department of Pathology & Laboratory Medicine, University of California, Los Angeles, California, USA
Infectious Disease Clinical Outcomes Research Unit, Division of Infectious Disease, Los Angeles Biomedical Research Institute, Harbor-UCLA Medical Center, Torrance, California, USA Torrance Memorial Medical Center, Torrance, California, USA.
出版信息
J Clin Microbiol. 2015 Dec;53(12):3712-4. doi: 10.1128/JCM.02668-15. Epub 2015 Oct 14.
Abstract
Detecting carbapenem-resistant Enterobacteriaceae (CRE) can be difficult. In particular, the absence of FDA-cleared automated antimicrobial susceptibility test (AST) devices that use revised Clinical and Laboratory Standards Institute (CLSI) carbapenem breakpoints for Enterobacteriaceae and the lack of active surveillance tests hamper the clinical laboratory. In this issue of the Journal of Clinical Microbiology, Lau and colleagues (A. F. Lau, G. A. Fahle, M. A. Kemp, A. N. Jassem, J. P. Dekker, and K. M. Frank, J Clin Microbiol 53:3729-3737, 2015, http://dx.doi.org/10.1128/JCM.01921-15) evaluate the performance of a research-use-only PCR for the detection of CRE in rectal surveillance specimens.
摘要
检测耐碳青霉烯类肠杆菌科细菌（CRE）可能具有挑战性。特别是，缺乏经美国食品药品监督管理局（FDA）批准的、使用修订后的临床和实验室标准协会（CLSI）针对肠杆菌科细菌的碳青霉烯类药物折点的自动化抗菌药物敏感性试验（AST）设备，以及缺乏主动监测检测方法，这给临床实验室带来了阻碍。在本期《临床微生物学杂志》中，Lau及其同事（A.F. Lau、G.A. Fahle、M.A. Kemp、A.N. Jassem、J.P. Dekker和K.M. Frank，《临床微生物学杂志》53:3729 - 3737，2015年，http://dx.doi.org/10.1128/JCM.01921 - 15）评估了一种仅供研究使用的PCR方法在直肠监测标本中检测CRE的性能。
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