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使用含肉汤增菌的显色培养基改善对耐碳青霉烯类肠杆菌科细菌的监测。

Improved surveillance for carbapenem-resistant Enterobacteriaceae using chromogenic media with a broth enrichment.

作者信息

Glaser Laurel, Andreacchio Kathleen, Lyons Mathilda, Alby Kevin

机构信息

Clinical Microbiology Laboratory, Hospital of the University of Pennsylvania, Philadelphia, PA, 19104; Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104.

Clinical Microbiology Laboratory, Hospital of the University of Pennsylvania, Philadelphia, PA, 19104.

出版信息

Diagn Microbiol Infect Dis. 2015 Aug;82(4):284-5. doi: 10.1016/j.diagmicrobio.2015.03.011. Epub 2015 Mar 18.

Abstract

In this study, 238 rectal screening swabs for carbapenem-resistant Enterobacteriaceae (CRE) were compared using chromID CARBA plates and the CDC reference method. Direct inoculation of chromID CARBA plates only identified 53% of CRE, whereas meropenem broth enrichment increased the positive agreement to 88% and identified additional cases of CRE. Additionally, the chromID CARBA method allowed for faster results and decreased the number of colonies further tested.

摘要

在本研究中,使用chromID CARBA平板和美国疾病控制与预防中心(CDC)参考方法对238份用于检测耐碳青霉烯类肠杆菌科细菌(CRE)的直肠筛查拭子进行了比较。直接接种chromID CARBA平板仅鉴定出53%的CRE,而美罗培南肉汤增菌使阳性一致性提高到88%,并鉴定出更多CRE病例。此外,chromID CARBA方法能更快得出结果,并减少了进一步检测的菌落数量。

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