Department of Clinical Laboratory, Obstetrics and Gynecology Hospital of Fudan University, No. 419 Fangxie Road, Shanghai, 200011, China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, 200011, China
Department of Clinical Laboratory, Obstetrics and Gynecology Hospital of Fudan University, No. 419 Fangxie Road, Shanghai, 200011, China.
Med Mycol. 2016 May;54(4):394-9. doi: 10.1093/mmy/myv082. Epub 2015 Oct 13.
In our multicenter study, we studied the distribution of Candida species in vulvovaginal candidiasis patients and investigated antifungal susceptibility profile and genotype of Candida albicans in vaginal swab. A total of 115 Candida albicans strains were detected in 135 clinical isolates. Minimum inhibitory concentration determinations showed that 83% and 81% of the 115 Candida albicans strains were susceptible to fluconazole and voriconazole. Randomly amplified polymorphic DNA analysis (RAPD) was applied to identify clonally related isolates from different patients at the local level. All tested strains were classified into genotype A (77.4%), genotype B (18.3%), and genotype C (4.3%). Genotype A was further classified into five subtypes and genotype B into two subtypes.Candida albicans was the dominant pathogen of vulvovaginal candidiasis, the majority belonging to genotype A in this study. Exposure to azoles is a risk factor for the emergence of azole resistance among Candida albicans isolated from VVC patients.
在我们的多中心研究中，我们研究了外阴阴道念珠菌病患者中念珠菌的分布，并调查了阴道拭子中念珠菌属白念珠菌的抗真菌药敏谱和基因型。在 135 例临床分离株中检测到 115 株白念珠菌。最低抑菌浓度测定显示，115 株白念珠菌中有 83%和 81%对氟康唑和伏立康唑敏感。随机扩增多态性 DNA 分析（RAPD）应用于鉴定来自不同患者的局部克隆相关分离株。所有测试菌株均分为基因型 A（77.4%）、基因型 B（18.3%）和基因型 C（4.3%）。基因型 A 进一步分为 5 个亚型，基因型 B 分为 2 个亚型。白念珠菌是外阴阴道念珠菌病的主要病原体，本研究中大多数属于基因型 A。唑类药物暴露是导致 VVC 患者分离的白念珠菌出现唑类耐药的危险因素。
