Amanloo Saeid, Zanjani Masoomeh, Serajian Sahar, Ahmadi Farzaneh, Kakavand Firoozeh
Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Medical Laboratory Sciences, School of Paramedical Sciences, Zanjan University of Medical Sciences, Zanjan, Iran.
Curr Med Mycol. 2022 Dec;8(4):9-14. doi: 10.32598/CMM.2023.1364.
Genotyping of pathogenic microorganisms is important for epidemiological studies and the adoption of appropriate strategies to control infectious diseases. In this regard, the present study aimed to genotype strains isolated from vulvovaginal candidiasis (VVC) patients using combined ABC type (25SrDNA) and repetitive sequence (RPS) typing systems. using combined typing systems of ABC type (25SrDNA) and repetitive sequence (RPS).
In total, 140 patients with VVC were investigated. Vaginal discharges were collected on Sabouraud dextrose agar and identified by CHROMagar. After species identification, a polymerase chain reaction system targeting 25S rDNA as well as ALT repeats in the RPS was designed to determine genotypes. The dendrogram was constructed by zero-one matrix data based on the combination of ABC and RPS typing systems. Statistical analysis of data was performed in SPSS software (version 23).
In total, 41 (29.3%) isolates were obtained from 140 VVC patients. The most common species that were identified included (56.1%) and (39%). Genotype A3 with five isolates (31.25%) had the highest frequency, followed by B2/3 with three isolates (18.3%), A3/4, C3/4, and B3/4 with two isolates (12.5%), and C2/3 and C3 with one isolate (6.25%), respectively. No significant association was found between the genotypes and antifungal resistance (<0.05).
The results showed that non- species are more prevalent in VVC patients, compared to . The results also indicated that ABC and RPS typings are useful for rapid genotyping and differentiation of isolates in regional and small-scale studies.
致病微生物的基因分型对于流行病学研究以及采取适当策略控制传染病至关重要。在这方面，本研究旨在使用ABC型（25SrDNA）和重复序列（RPS）分型系统联合对从外阴阴道念珠菌病（VVC）患者分离出的菌株进行基因分型。使用ABC型（25SrDNA）和重复序列（RPS）联合分型系统。
共调查了140例VVC患者。在沙氏葡萄糖琼脂上收集阴道分泌物并通过科玛嘉显色培养基进行鉴定。在菌种鉴定后，设计了一种针对25S rDNA以及RPS中ALT重复序列的聚合酶链反应系统来确定基因型。基于ABC和RPS分型系统的组合，通过零一矩阵数据构建树状图。在SPSS软件（版本23）中进行数据的统计分析。
从140例VVC患者中共获得41株（29.3%）分离株。鉴定出的最常见菌种包括（56.1%）和（39%）。基因型A3有5株（31.25%），频率最高，其次是B2/3有3株（18.3%），A3/4、C3/4和B3/4各有2株（12.5%），C2/3和C3各有1株（6.25%）。基因型与抗真菌耐药性之间未发现显著关联（<0.05）。
结果表明，与相比，非菌种在外阴阴道念珠菌病患者中更为普遍。结果还表明，ABC和RPS分型对于区域和小规模研究中念珠菌分离株的快速基因分型和鉴别是有用的。
