Eisenbrey A B, Epstein E, Zak B, McEnroe R J, Artiss J D, Kiechle F L
Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, Michigan 48072.
Am J Clin Pathol. 1989 Mar;91(3):293-7. doi: 10.1093/ajcp/91.3.293.
Phosphatidylglycerol (PG) in amniotic fluid is recognized as a good indicator of fetal lung maturity and is unaffected by moderate amounts of blood or meconium contamination. A rapid immunologic agglutination assay, Ultrasensitive AmnioStat-FLM (FLM), was compared with two-dimensional thin-layer chromatography (TLC) and an enzymic, colorimetric procedure (E-PG). Eighty amniotic fluid specimens were analyzed. FLM results were reported as high (H), intermediate (I), or low positive (L). TLC was compared with FLM:H (n = 27), mean 0.14 (fraction of total phospholipids); I (n = 7), mean 0.11; L (n = 9), mean 0.03; negative results had no detectable PG by TLC. In 33 cases E-PG was compared with FLM:H (n = 9), mean 7.0 mumol/L; I (n = 5), mean 8.1 mumol/L; L (n = 3), mean 3.0 mumol/L; negative (n = 16), mean 3.2 mumol/L. Records were reviewed in 70 cases. Thirty cases were excluded: sample to delivery time was greater than 72 hours; steroids were given or sepsis was documented. Fetal lung immaturity was clinically present in six cases: respiratory distress syndrome in three cases and transient tachypnea of the newborn (TTN) in three cases. One false positive result was identified (TTN, FLM:H). FLM sensitivity for fetal lung maturity was 85.3%, specificity was 83.3%, and the positive predictive value for fetal lung maturity was 96.7%. FLM is a fast, reliable indicator of fetal lung maturity.
羊水磷脂酰甘油（PG）被认为是胎儿肺成熟度的良好指标，且不受适量血液或胎粪污染的影响。将一种快速免疫凝集试验——超敏羊水胎儿肺成熟度检测法（FLM）与二维薄层色谱法（TLC）及一种酶促比色法（E-PG）进行了比较。分析了80份羊水标本。FLM结果报告为高（H）、中（I）或低阳性（L）。将TLC与FLM进行比较：H（n = 27），平均0.14（占总磷脂的比例）；I（n = 7），平均0.11；L（n = 9），平均0.03；阴性结果经TLC检测未发现PG。在33例中，将E-PG与FLM进行比较：H（n = 9），平均7.0 μmol/L；I（n = 5），平均8.1 μmol/L；L（n = 3），平均3.0 μmol/L；阴性（n = 16），平均3.2 μmol/L。回顾了70例的记录。排除30例：样本采集至分娩时间大于72小时；给予了类固醇或记录有败血症。临床上有6例存在胎儿肺不成熟：3例发生呼吸窘迫综合征，3例发生新生儿短暂性呼吸急促（TTN）。发现1例假阳性结果（TTN，FLM：H）。FLM对胎儿肺成熟度的敏感性为85.3%，特异性为83.3%，对胎儿肺成熟度的阳性预测值为96.7%。FLM是一种快速、可靠的胎儿肺成熟度指标。
