Coapman-Hankin R A, Kiechle F L, Epstein E, Artiss J D, Zak B
Clin Chem. 1985 Aug;31(8):1374-6.
Phosphatidylglycerol is one component of amniotic fluid that is unaffected by several of the reported interferences with conventional thin-layer chromatographic measurement of phospholipid. To compare a rapid immunological agglutination test, "Amniostat-FLM", with two-dimensional thin-layer chromatography, we determined phosphatidylglycerol by both methods in 41 amniotic fluid specimens obtained at 31 to 40 weeks of gestation. We also assayed phosphatidylglycerol in 14 of these specimens by an enzymic, colorimetric procedure. The agglutination test is rapid and simple but relatively insensitive; it yielded positive results for only seven of 23 specimens in which phosphatidylglycerol was detected by thin-layer chromatography. In 18 specimens in which no phosphatidylglycerol was detected by thin-layer chromatography, results by Amniostat-FLM were also negative; however, eight of these specimens, assayed by the enzymic method, had phosphatidylglycerol present. Apparently, the Amniostat-FLM detects phosphatidylglycerol only at concentrations exceeding 25 mumol/L, or more than 15% of the total phospholipid composition.
磷脂酰甘油是羊水的一种成分，不受几种已报道的对传统薄层色谱法测定磷脂的干扰因素影响。为了将快速免疫凝集试验“羊水胎儿肺成熟度检测试剂盒（Amniostat-FLM）”与二维薄层色谱法进行比较，我们用这两种方法对41例妊娠31至40周时获取的羊水标本中的磷脂酰甘油进行了测定。我们还用酶比色法对其中14份标本中的磷脂酰甘油进行了检测。凝集试验快速简便，但相对不敏感；在23份通过薄层色谱法检测出磷脂酰甘油的标本中，该试验仅对7份标本得出了阳性结果。在18份通过薄层色谱法未检测出磷脂酰甘油的标本中，羊水胎儿肺成熟度检测试剂盒（Amniostat-FLM）的检测结果也为阴性；然而，用酶法检测的这些标本中有8份存在磷脂酰甘油。显然，羊水胎儿肺成熟度检测试剂盒（Amniostat-FLM）仅在磷脂酰甘油浓度超过25 μmol/L或占总磷脂成分超过15%时才能检测到。
