College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601 Hefei, PR China.
School of Life Science, Anhui University, Hefei 230601, PR China.
Biosens Bioelectron. 2016 Mar 15;77:530-6. doi: 10.1016/j.bios.2015.09.059. Epub 2015 Nov 14.
Two novel similar structural triphenylamine-based Schiff base fluorescent probes (L1/L2) were designed, prepared and characterized. Distinctive recognition mechanisms of L1 and L2 toward Al(3+) and Zn(2+) have been established by UV/vis, fluorescence spectra, mass spectra and (1)H NMR studies, respectively. To further explore their utility in biological system, L2 was selected as a probe for live cell endogenous Zn(2+) indicator and showed superb sensitivity on Zn(2+) intracellular distribution. Furthermore, L2 was employed to selectively detect Zn(2+) in live tissues at both extracellular and intracellular level, qualitatively indicated varies zinc concentration as a function of different organs.
两种新型结构类似的三苯胺基席夫碱荧光探针（L1/L2）被设计、合成并进行了结构表征。通过紫外可见吸收光谱、荧光光谱、质谱和氢核磁共振谱等手段分别研究了探针 L1 和 L2 对 Al(3+)和 Zn(2+)的识别机理。为了进一步探索它们在生物体系中的应用，选择 L2 作为活细胞内源性 Zn(2+)指示剂的探针，对 Zn(2+)在细胞内的分布具有很好的灵敏度。此外，L2 被用于选择性地检测活组织中的 Zn(2+)，包括细胞外和细胞内水平，并定性地表明不同器官的锌浓度随功能的不同而变化。
