Institute of Functional Organic Molecular Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, 475004, China.
Analyst. 2019 Oct 7;144(19):5706-5716. doi: 10.1039/c9an01295h. Epub 2019 Aug 22.
A novel Schiff base fluorescence probe (HL) was synthesized by the condensation of salicylaldehyde and an 8-aminoquinoline derivative. This probe acts as a "turn-on" dual selectivity fluorescence probe for Zn and Al ions, providing different colors and detection limits (DL) of 11.5 and 23.5 nM, respectively. Moreover, when Zn and Al co-exist, HL exhibits a preference for Al by displacing Zn from the HL-Zn complex, realizing a dual-channel signal output for Al. The HL-Al system could further discern F by a "turn-off" fluorescence response with a DL of 86.0 nM. Furthermore, the probe HL was capable of monitoring intracellular Al, Zn and F in living PC12 cells in vitro through fluorescence imaging, which proved its value in potential in vivo applications.
一种新型希夫碱荧光探针（HL）是由水杨醛和 8-氨基喹啉衍生物缩合而成的。该探针可作为 Zn 和 Al 离子的“开启”型双选择性荧光探针，其检测限（DL）分别为 11.5 和 23.5 nM，呈现出不同的颜色。此外，当 Zn 和 Al 共存时，HL 优先与 Al 结合，从而从 HL-Zn 配合物中置换出 Zn，实现了 Al 的双通道信号输出。HL-Al 体系还可以通过“关闭”荧光响应进一步区分 F，其 DL 为 86.0 nM。此外，探针 HL 能够通过荧光成像在体外监测活 PC12 细胞内的 Al、Zn 和 F，这证明了其在潜在的体内应用中的价值。
