Xia Huika, Nan Yan, Huang Xin, Gao Jie, Pu Mingliang
Department of Anatomy/Embryology School of Basic Medical Sciences, Peking University, Beijing, China 2Key Laboratory on Machine Perception (Ministry of Education), Peking University, Beijing, China 3Key Laboratory for Visual Impairment and Restoration (Mi.
Invest Ophthalmol Vis Sci. 2015 Oct;56(11):6638-45. doi: 10.1167/iovs.15-17301.
To investigate the effects of tauroursodeoxycholic acid (TUDCA) and alpha-lipoic-acid (ALA) on the visual response properties of cat retinal ganglion cells (RGCs) in wholemount retinas.
Young adult cats were divided into three groups: control, ALA, and TUDCA. In vitro single-unit extracellular recordings were performed on wholemount retinas to objectively evaluate the visual response properties of RGCs prior and post to antioxidant treatment. The visual responses properties of RGCs, including receptive field size, luminance threshold, and contrast sensitivity, were collected online and analyzed off-line with Axon Pclamp9.
Most of the RF sizes were larger than those plotted prior to the 60 minutes dark adaptation. The luminance threshold was elevated in the control group (no treatment) but reduced post ALA treatment and significantly reduced post TUDCA treatment. The contrast threshold was significantly elevated in the control group (no treatment) and clearly elevated post ALA treatment but effectively sustained post TUCDA treatment.
Retinal neurocircuitry deteriorates in wholemount retinas, resulting in abnormal visual response properties in RGCs. Alpha-lipoic-acid and TUDCA exerted beneficial neuroprotective effects by activating the antioxidant pathway, partially restoring the functionality of retinal neurocircuitry and significantly improving the visual response properties of RGCs. However, TUDCA appears to be more effective than ALA in reducing irradiance thresholds and improving contrast sensitivity.
研究牛磺熊去氧胆酸（TUDCA）和α-硫辛酸（ALA）对猫视网膜全层神经节细胞（RGCs）视觉反应特性的影响。
将成年幼猫分为三组：对照组、ALA组和TUDCA组。对视网膜全层进行体外单单位细胞外记录，以客观评估抗氧化治疗前后RGCs的视觉反应特性。在线收集RGCs的视觉反应特性，包括感受野大小、亮度阈值和对比度敏感度，并使用Axon Pclamp9进行离线分析。
大多数感受野大小比暗适应60分钟前绘制的更大。对照组（未治疗）的亮度阈值升高，但ALA治疗后降低，TUDCA治疗后显著降低。对照组（未治疗）的对比度阈值显著升高，ALA治疗后明显升高，但TUDCA治疗后有效维持。
视网膜全层中的神经回路退化，导致RGCs的视觉反应特性异常。α-硫辛酸和TUDCA通过激活抗氧化途径发挥有益的神经保护作用，部分恢复视网膜神经回路的功能，并显著改善RGCs的视觉反应特性。然而，TUDCA在降低光照阈值和提高对比度敏感度方面似乎比ALA更有效。
