Wang Shuo, Gao Cheng-Wen, Gao Li-Zhi
a Faculty of Environmental Science and Engineering and.
b Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4407-4408. doi: 10.3109/19401736.2015.1089546. Epub 2015 Oct 15.
Here we assembled and analyzed the complete chloroplast genome of Prunus mume, a popular ornamental and editable fruit tree of Rosaceae. The cp genome exhibited a circular DNA molecule of 157 712 bp with a typical quadripartite structure consisted of two inverted repeat regions (IRa and IRb) of 26 394 bp separated by large (LSC) and small (SSC) single-copy regions of 85 861 and 19 063 bp, respectively. It encoded 112 unique genes, 19 of which were duplicated in the IR regions, giving a total of 131 genes. Eighteen of these genes harbored one or two introns. GC content was 38.9%, and coding regions accounted for 51.3% of the genome. Phylogenetic analysis showed that P. mume clustered with P. persica and P. kansuensis in the genus Punus. This newly determined chloroplast genome will enhance modern breeding programs for the purpose of genetic improvement of this valuable plant.
我们在此组装并分析了梅花的完整叶绿体基因组，梅花是蔷薇科一种广受欢迎的观赏兼食用果树。叶绿体基因组呈现出一个157712 bp的环状DNA分子，具有典型的四分体结构，由两个26394 bp的反向重复区域（IRa和IRb）组成，这两个区域被分别为85861 bp和19063 bp的大单拷贝区域（LSC）和小单拷贝区域（SSC）隔开。它编码112个独特基因，其中19个在IR区域重复，共计131个基因。这些基因中有18个含有一或两个内含子。GC含量为38.9%，编码区域占基因组的51.3%。系统发育分析表明，梅花与李属的桃和甘肃桃聚类在一起。这个新确定的叶绿体基因组将推动旨在对这种珍贵植物进行遗传改良的现代育种计划。
