College of Forestry, Inner Mongolia Agricultural University, Hohhot 010018, China.
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Non-Timber Forestry, Chinese Academy of Forestry, Zhengzhou 450003, China.
Genes (Basel). 2023 Oct 18;14(10):1959. doi: 10.3390/genes14101959.
The production and quality of apricots in China is currently limited by the availability of germplasm resource characterizations, including identification at the species and cultivar level. To help address this issue, the complete chloroplast genomes of L., L. and kernel consumption apricot were sequenced, characterized, and phylogenetically analyzed. The three chloroplast (cp) genomes ranged from 157,951 to 158,224 bp, and 131 genes were identified, including 86 protein-coding genes, 37 rRNAs, and 8 tRNAs. The GC content ranged from 36.70% to 36.75%. Of the 170 repetitive sequences detected, 42 were shared by all three species, and 53-57 simple sequence repeats were detected with AT base preferences. Comparative genomic analysis revealed high similarity in overall structure and gene content as well as seven variation hotspot regions, including , , , , , , and . Phylogenetic analysis showed that the three apricot species clustered into one group, and the genetic relationship between and kernel consumption apricot was the closest. The results of this study provide a theoretical basis for further research on the genetic diversity of apricots and the development and utilization of molecular markers for the genetic engineering and breeding of apricots.
中国的杏生产和质量目前受到种质资源特性的限制，包括在物种和品种水平上的鉴定。为了帮助解决这个问题，我们对 、 和仁用杏的完整叶绿体基因组进行了测序、特征描述和系统发育分析。这三个叶绿体基因组的长度范围为 157951 到 158224bp，共鉴定出 131 个基因，包括 86 个蛋白编码基因、37 个 rRNA 和 8 个 tRNA。GC 含量范围为 36.70%到 36.75%。在检测到的 170 个重复序列中，有 42 个被三种物种共有，有 53-57 个简单序列重复，具有 AT 碱基偏好性。比较基因组分析显示，这三种杏在整体结构和基因组成上具有高度的相似性，并且存在七个变异热点区域，包括 、 、 、 、 、和 。系统发育分析表明，这三个杏种聚为一组，与仁用杏的遗传关系最为密切。本研究结果为进一步研究杏的遗传多样性以及杏的遗传工程和育种中分子标记的开发和利用提供了理论基础。
