Zhang X, Tang B, Hou Y
Fujian Provincial Key Laboratory of Insect Ecology, Department of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China. Key Laboratory of Integrated Pest Management on Crops in Fujian-Taiwan, Ministry of Agriculture, P. R. China. These two authors contributed equally to the work.
Fujian Provincial Key Laboratory of Insect Ecology, Department of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China. Key Laboratory of Integrated Pest Management on Crops in Fujian-Taiwan, Ministry of Agriculture, P. R. China.
J Econ Entomol. 2015 Feb;108(1):95-9. doi: 10.1093/jee/tou025. Epub 2015 Jan 24.
Octodonta nipae (Maulik) is morphologically and biologically similar to Brontispa longissima (Gestro), one of the most damaging pests of coconut. The two species share several palm hosts and produce similar symptoms. They are easily confused when they invade a new area, and without professional taxonomic expertise accurate identification is arduous and time-consuming. Thus, a method of rapidly distinguishing these two invasive insects rapidly is critical for quarantine. Based on the first internal transcribed spacer and the mitochondrial cytochrome oxidase I gene, two pairs of special primers and corresponding polymerase chain reaction processes have been developed to enhance a single objective band only from the O. nipae DNA template. This will quickly discriminate between these two species. The present results provide a rapid method of distinguishing O. nipae from B. longissima to help avoid misidentification, and furthermore, to facilitate rapid and appropriate quarantine decisions and effective treatments to the pest.
椰蛀犀金龟（Maulik）在形态和生物学上与椰心叶甲（Gestro）相似，椰心叶甲是椰子最具危害性的害虫之一。这两个物种共有几种棕榈寄主，并产生相似的症状。当它们侵入新区域时很容易混淆，并且没有专业的分类学专业知识，准确识别既艰巨又耗时。因此，一种快速区分这两种入侵昆虫的方法对于检疫至关重要。基于第一个内部转录间隔区和线粒体细胞色素氧化酶I基因，已经开发出两对特殊引物和相应的聚合酶链反应过程，以仅从椰蛀犀金龟DNA模板中增强单一目标条带。这将快速区分这两个物种。目前的结果提供了一种快速区分椰蛀犀金龟和椰心叶甲的方法，有助于避免错误识别，此外，有助于对该害虫做出快速和适当的检疫决定并进行有效处理。
