Chen Zhiming, Chen Jun, Zhang Xiang, Hou Youming, Wang Guihua
State Key Laboratory of Ecological Pest Control of Fujian-Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Fujian Provincial Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Can J Infect Dis Med Microbiol. 2018 Jun 7;2018:9139306. doi: 10.1155/2018/9139306. eCollection 2018.
The nipa palm hispid beetle, (Maulik) (Coleoptera: Chrysomelidae), is an important invasive pest on palm plants in southern China. Based on existing transcriptome data, polymorphism simple sequence repeat (SSR) loci were identified. In total, 1274 SSR loci were identified from 49919 unigenes. The majority of them contained mononucleotide, dinucleotide, and trinucleotide motifs (43.56%, 26.14%, and 28.18%), in which A/T (41.21%) and AT/TA (15.86%) were the most abundant motifs. 104 pairs of the SSR primers produced amplification bands of expected sizes in , 80 pairs of SSR primers were tested randomly for polymorphism, 9 loci of them were validated to be polymorphic markers, and the number of alleles ranged from 2 to 3, with an average of 2.56 per locus. The population of Zhangzhou and Fuzhou was analyzed by the 9 loci (On1-On9). These SSR transcriptome data can provide invaluable resource for SSR development, population genetics research, invasion and expansion mechanism, paternity testing, and other research on and its related species.
尼帕榈粗角萤叶甲（Maulik）（鞘翅目：叶甲科）是中国南方棕榈植物上的一种重要入侵害虫。基于现有的转录组数据，鉴定了多态性简单序列重复（SSR）位点。总共从49919个单基因中鉴定出1274个SSR位点。其中大多数包含单核苷酸、二核苷酸和三核苷酸基序（分别为43.56%、26.14%和28.18%），其中A/T（41.21%）和AT/TA（15.86%）是最丰富的基序。104对SSR引物在[具体物种]中产生了预期大小的扩增条带，随机测试了80对SSR引物的多态性，其中9个位点被验证为多态性标记，等位基因数范围为2至3，每个位点平均为2.56个。利用这9个位点（On1-On9）对漳州和福州的种群进行了分析。这些SSR转录组数据可为SSR开发、种群遗传学研究、入侵和扩散机制、亲子鉴定以及对该物种及其相关物种的其他研究提供宝贵资源。
