Li Weidi, Zhang Jingming, Zhang Pengjun, Lin Wencai, Lin Qingsheng, Li Zhenyu, Hang Fang, Zhang Zhijun, Lu Yaobin
Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Hangzhou 310021, China.
Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Hangzhou 310021, China. Corresponding author, e-mail:
J Econ Entomol. 2015 Apr;108(2):736-41. doi: 10.1093/jee/tou060. Epub 2015 Jan 25.
Spinetoram is a spinosyn, which is a unique class of natural insecticide. Because of its novel mode of action, spinetoram is more potent and faster acting than other insecticides, even the older spinosyn product, spinosad. On account of being efficient on insect order Lepidoptera, spinetoram provides a new alternative for control of Plutella xylostella (L.) (Lepidoptera: Plutellidae), which are resistant to other chemicals. To determine the current situation of resistance of P. xylostella to spinetoram, the susceptibility of 16 P. xylostella populations from different regions of China or different time in addition to the population from laboratory was assessed using a leaf dip bioassay. The variation in spinetoram susceptibility among the 16 field populations was narrow, with median lethal concentrations (LC50 values) ranging from 0.131 to 1.001 mg/liter. Toxicity ratios (TRs) ranged from 1.5 to 7.6 and were 5.6 and 7.6 for populations SY-2 and FX-1, respectively, indicating some low level of tolerance in these populations. A discriminating concentration (a concentration that can detect the occurrence of resistance in a population) of 10 mg/liter, which was identified based on the pooled toxicological data, caused 100% mortality in all nine tested populations. The baseline susceptibility data reflect the natural variation of the P. xylostella populations to spinetoram rather than variation caused by previous exposure.
多杀霉素是一种多杀菌素，属于一类独特的天然杀虫剂。由于其独特的作用方式，多杀霉素比其他杀虫剂甚至是较早的多杀菌素产品多杀霉素更有效且起效更快。鉴于对鳞翅目昆虫高效，多杀霉素为防治对其他化学药剂产生抗性的小菜蛾（鳞翅目：菜蛾科）提供了一种新的选择。为了确定小菜蛾对多杀霉素的抗性现状，除了实验室种群外，还使用叶片浸渍生物测定法评估了来自中国不同地区或不同时间的16个小菜蛾种群的敏感性。16个田间种群对多杀霉素的敏感性差异较小，半数致死浓度（LC50值）范围为0.131至1.001毫克/升。毒性比（TRs）范围为1.5至7.6，SY-2和FX-1种群的毒性比分别为5.6和7.6，表明这些种群存在一定程度的低水平耐受性。根据汇总的毒理学数据确定的10毫克/升的区分浓度（可检测种群中抗性发生情况的浓度）在所有9个测试种群中均导致100%的死亡率。基线敏感性数据反映了小菜蛾种群对多杀霉素的自然变异，而非先前接触所导致的变异。
