College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects of Ministry of Agriculture, Nanjing 210095, China.
J Econ Entomol. 2010 Jun;103(3):843-8. doi: 10.1603/EC09367.
Diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), has evolved resistance to various kinds of insecticides in the field. Chlorantraniliprole, which is a new pesticide of the anthranilic diamide group, provides potent and broad-spectrum activity within the insect order Lepidoptera. This insecticide provides a new option for control of P. xylostella populations resistant to other chemicals. The susceptibility of 16 field populations and seven laboratory maintained strains of P. xylostella to chlorantraniliprole were determined through leaf dip bioassay. The susceptibility variation among 16 field populations was low (five-fold), with median lethal concentrations (LC50 values) varying from 0.221 to 1.104 mg/liter. However, wider ranges of variation in LC50 values (10-fold) were observed among seven laboratory strains. Low level tolerance (six- to 10-fold) was detected in two laboratory-selected strains and three field-collected populations when compared with the susceptible Roth strain. A discriminating concentration (15 mg/liter) was calibrated from pooled toxicological data of the 16 field populations, as an important first step in resistance management, for the routine monitoring of resistance in the future. When assessed at the established discriminating dose 15 mg/liter, seven laboratory strains and five field populations showed an average mortality of 99.75% (from 98 to 100%). Synergism assays showed metabolic enzymes might be involved in chlorantraniliprole detoxification in the susceptible Roth strain, but not in the additional observed tolerance of strains selected for resistance with other insecticides.
小菜蛾（Plutella xylostella（L.））（鳞翅目：菜蛾科）在田间已对各种杀虫剂产生了抗药性。氯虫苯甲酰胺是一种新型邻苯二甲酰胺类杀虫剂，对鳞翅目昆虫具有高效广谱的活性。这种杀虫剂为防治对其他化学物质具有抗药性的小菜蛾种群提供了一种新的选择。通过叶浸生物测定法测定了 16 个田间种群和 7 个实验室饲养的小菜蛾种群对氯虫苯甲酰胺的敏感性。16 个田间种群的敏感性差异较低（五倍），半数致死浓度（LC50 值）在 0.221 至 1.104 毫克/升之间。然而，在 7 个实验室菌株中观察到 LC50 值的变化范围更宽（十倍）。与敏感的 Roth 菌株相比，在两个实验室选择的菌株和三个田间采集的种群中检测到低水平的耐受性（六到十倍）。从 16 个田间种群的综合毒理学数据中校准出一个区分浓度（15 毫克/升），作为抗性管理的重要第一步，用于未来常规监测抗性。在设定的区分剂量 15 毫克/升下评估时，七个实验室菌株和五个田间种群的平均死亡率为 99.75%（98%至 100%）。增效作用试验表明，代谢酶可能参与了敏感的 Roth 菌株中氯虫苯甲酰胺的解毒作用，但在其他杀虫剂选择的抗性菌株中观察到的额外耐受性中则没有参与。
