七星瓢虫（脉翅目：草蛉科）卵黄原蛋白通过影响产卵和孵化发挥作用。
Chrysopa septempunctata (Neuroptera: Chrysopidae) Vitellogenin Functions Through Effects on Egg Production and Hatching.
作者信息
Liu C, Mao J, Zeng F
机构信息
Key Laboratory for Biology of Plant Diseases and Insect Pests, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. Both authors contributed equally to this work.
Key Laboratory for Biology of Plant Diseases and Insect Pests, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Both authors contributed equally to this work.
出版信息
J Econ Entomol. 2015 Dec;108(6):2779-88. doi: 10.1093/jee/tov229. Epub 2015 Aug 5.
Vitellogenin (Vg) is a precursor of major egg storage protein, vitellin (Vt), and plays primary roles in reproduction of oviparous vertebrates and invertebrates. Chrysopa septempunctata Wesmael is an important and common predator of various insect pests. Here, we first cloned C. septempunctata Vg gene, CsVg. The complete CsVg cDNA was 5664 bp, which encodes an 1810-residues protein with a predicted molecular mass of 206.23 kDa. Expression profile revealed that CsVg mRNA first appeared on day 4 after emergence, maximally accumulated on day 10, and then declined gradually. RNAi mediated by injection of dsRNA depleted CsVg transcripts, significantly reduced egg-laying amount, and decreased egg hatching rate, suggesting that CsVg functions through effects on egg production and hatching in C. septempunctata.
卵黄原蛋白（Vg）是主要的卵储存蛋白卵黄磷蛋白（Vt）的前体，在卵生脊椎动物和无脊椎动物的繁殖中起主要作用。七星瓢虫是多种害虫的重要常见捕食者。在此，我们首次克隆了七星瓢虫的Vg基因CsVg。CsVg cDNA全长5664bp，编码一个1810个氨基酸的蛋白质，预测分子量为206.23kDa。表达谱显示，CsVg mRNA在羽化后第4天首次出现，在第10天积累到最大值，然后逐渐下降。注射dsRNA介导的RNA干扰使CsVg转录本减少，显著降低产卵量，并降低卵孵化率，这表明CsVg通过影响七星瓢虫的产卵和孵化发挥作用。
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