Terry G M, Ho-Terry L, Londesborough P, Rees K R
Department of Chemical Pathology, Faculty of Clinical Sciences, University College, London, England.
Arch Virol. 1989;104(1-2):63-75. doi: 10.1007/BF01313808.
The major rubella envelope protein, E1, and a segment of it, comprising amino acids 207-353, have been separately expressed as fusion proteins with the IgG binding region of Staphylococcus aureus protein A in Escherichia coli. The proteins carry E1-specific antigenicity recognized by monoclonal antibodies raised against whole virus confirming that correct glycosylation is not required for antigenicity. The use of these bioengineered antigens in immunoassays for diagnosis of rubella infection and for immunization in experimental animals is described.
风疹主要包膜蛋白E1及其包含氨基酸207 - 353的片段，已分别在大肠杆菌中作为与金黄色葡萄球菌蛋白A的IgG结合区的融合蛋白表达。这些蛋白带有针对全病毒产生的单克隆抗体所识别的E1特异性抗原性，证实抗原性不需要正确的糖基化。本文描述了这些生物工程抗原在风疹感染诊断免疫测定及实验动物免疫中的应用。
