Saikia L, Sharma A, Nath R, Choudhury G, Borah A K
Department of Microbiology, Assam Medical College, Dibrugarh, Assam, India.
Indian J Med Microbiol. 2015 Oct-Dec;33(4):503-6. doi: 10.4103/0255-0857.167347.
Salmonella enterica serovar Weltevreden has been a rare cause of acute gastroenteritis occurring worldwide. Here, we report an outbreak of food poisoning in a tea garden.
To determine the aetiological agent and risk factors responsible for the outbreak and to take necessary steps for prevention of future outbreaks.
Affected area was visited by a team of microbiologists for collecting stool samples/rectal swabs from affected patients. Samples were processed by culture followed by confirmation of the isolates biochemically, automated bacterial identification system, conventional serotyping and molecular typing. Water samples were also processed for detection of faecal contamination. Antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion technique according to the Clinical Laboratory Standard Institute guidelines.
The isolates were confirmed as S. enterica subspecies enterica serovar Weltevreden. They were found sensitive to ampicillin, amoxycillin-clavulanic acid, ciprofloxacin, ofloxacin, norfloxacin, cefotaxime, ceftriaxone, co-trimoxazole and doxycycline. Water samples showed high-level faecal contamination. Source of outbreak was found to be drinking water contaminated with dead livestock. House to house visit was made for early diagnosis and treatment of the cases, awareness campaigning and chlorination of drinking water.
This report emphasises the geographical distribution of this organism in Assam. As S. Weltevreden is widely distributed in domestic animals, people should be made aware of immediate reporting of any unusual death among the livestock and their safe disposal which can significantly reduce the incidence of non-typhoidal salmonellosis in the country.
肠炎沙门氏菌韦尔泰弗里登血清型一直是全球范围内急性肠胃炎的罕见病因。在此，我们报告了茶园中的一起食物中毒暴发事件。
确定此次暴发的病原体和危险因素，并采取必要措施预防未来的暴发。
一组微生物学家前往受影响地区，从受影响患者那里采集粪便样本/直肠拭子。样本通过培养进行处理，随后通过生化方法、自动化细菌鉴定系统、传统血清分型和分子分型对分离株进行确认。还对水样进行处理以检测粪便污染情况。根据临床实验室标准协会的指南，采用 Kirby-Bauer 纸片扩散法进行药敏试验。
分离株被确认为肠炎沙门氏菌肠道亚种韦尔泰弗里登血清型。它们对氨苄西林、阿莫西林-克拉维酸、环丙沙星、氧氟沙星、诺氟沙星、头孢噻肟、头孢曲松、复方新诺明和强力霉素敏感。水样显示出高水平的粪便污染。发现暴发源是被死牲畜污染的饮用水。逐户走访以对病例进行早期诊断和治疗、开展宣传活动并对饮用水进行氯化处理。
本报告强调了该菌株在阿萨姆邦的地理分布情况。由于韦尔泰弗里登血清型在家畜中广泛分布，应让人们了解到牲畜出现任何异常死亡时需立即报告以及对其进行安全处理，这可显著降低该国非伤寒沙门氏菌病的发病率。
