Nafiu Abdulrazaq Bidemi, Rahman Mohammad Tariqur
Faculty of Science, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan, 25200, Pahang, Malaysia.
Faculty of Dentistry, University Malaya, Kuala Lumpur, 50603, Malaysia.
BMC Complement Altern Med. 2015 Oct 15;15:369. doi: 10.1186/s12906-015-0900-4.
Increased wound healing efficiency by Se(2+) added Carica papaya L. (Caricaceae) fruit extract was linked to increased antioxidant and anti-inflammatory responses during healing. We investigated the impact of Se(2+) or Zn(2+) added papaya water (WE) and phosphate-buffered saline (PE) extracts on cells recruitment and bio-molecular alterations on days 4 and 10 post wounding in an in vivo excision wound.
Excision wounds were created on the dorsum of Sprague Dawley rats and treated topically twice/day with 20 μL of PE and WE (5 mg extract/mL), 0.5 μgSe(2+) added PE and WE (PES and WES), or 100 μMZn(2+) added PE and WE (PEZ and WEZ). Deionised water (negative) and Solcoseryl (positive) were applied on the control groups. Histochemical and biochemical assays were used to evaluate cellular and bio-molecular changes in the wound.
PES (PE + 0.5 μg Se(2+)) only increased significantly (p < 0.05) wound total protein content (95.14 ± 1.15 mg/g tissue vs positive control; 80.42 ± 0.86 mg/g tissue) on day 10 post wounding. PES increased significantly (p < 0.05) the number of fibroblasts/high power field (HPF) (75.60 ± 9.66) but decreased significantly (p < 0.05) the number of polymorphonuclear leukocytes/HPF (59.20 ± 12.64) in the wound compared to positive control (50.60 ± 12.58 fibroblasts/HPF, 101.00 ± 27.99 polymorphonuclear leukocytes/HPF) on day 4. Similar results were recorded for WES. PES demonstrated increased neovascularization, TGF-β1 and VEGFA expressions at day 4 and increased collagen at day 10.
Papaya extract improved wound repair by increasing fibroblasts recruitment and reducing polymorphonuclear leukocytes infiltration through early transient expressions of TGF-β1 and VEGFA at the wound area. The processes were amplified with Se(2+) addition.
添加硒离子（Se(2+)）的番木瓜（番木瓜科）果实提取物可提高伤口愈合效率，这与愈合过程中抗氧化和抗炎反应增强有关。我们研究了添加Se(2+)或锌离子（Zn(2+)）的木瓜水提取物（WE）和磷酸盐缓冲盐水提取物（PE）对体内切除伤口愈合后第4天和第10天细胞募集和生物分子改变的影响。
在Sprague Dawley大鼠背部制造切除伤口，每天局部处理两次，每次20 μL的PE和WE（5 mg提取物/mL）、添加0.5 μg Se(2+)的PE和WE（PES和WES）或添加100 μM Zn(2+)的PE和WE（PEZ和WEZ）。对照组涂抹去离子水（阴性）和Solcoseryl（阳性）。采用组织化学和生化分析方法评估伤口中的细胞和生物分子变化。
在伤口愈合后第10天，PES（PE + 0.5 μg Se(2+)）仅显著增加（p < 0.05）伤口总蛋白含量（95.14 ± 1.15 mg/g组织，与阳性对照相比；80.42 ± 0.86 mg/g组织）。与阳性对照（50.60 ± 12.58个成纤维细胞/高倍视野，101.00 ± 27.99个多形核白细胞/高倍视野）相比，在伤口愈合后第4天，PES显著增加（p < 0.05）成纤维细胞/高倍视野（HPF）数量（75.60 ± 9.66），但显著减少（p < 0.05）多形核白细胞/HPF数量（59.20 ± 12.64）。WES记录到类似结果。在第4天，PES显示新生血管形成增加、TGF-β1和VEGFA表达增加，在第10天胶原蛋白增加。
木瓜提取物通过增加成纤维细胞募集和减少多形核白细胞浸润来改善伤口修复，这是通过伤口区域早期短暂表达TGF-β1和VEGFA实现的。添加Se(2+)可增强这些过程。
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