Li Kai, Xue Yang, Yan Ting, Zhang Lan, Han Yong
State-key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China.
Bioact Mater. 2020 Jan 25;5(1):116-123. doi: 10.1016/j.bioactmat.2020.01.001. eCollection 2020 Mar.
An ideal intraosseous transcutaneous implant should form a tight seal with soft tissue, besides a requirement of osseointegration at the bone-fixed position. Si substituted hydroxyapatite (Si-HA) nanorods releasing Si ion and simulating nanotopography of natural tissue were designed on Ti to enhance fibroblast response and biosealing with soft tissue . Si-HA nanorods were fabricated by alkali-heat treatment followed with hydrothermal treatment. The hydrothermal formation mechanism of Si-HA nanorods was explored. The surface characteristic of Si-HA nanorods was compared with pure HA nanorods. Fibroblast behaviors and skin response on different surfaces were also evaluated. The obtained results show that the substitution of Si did not significantly alter the phase component, morphology, roughness and wettability of HA, but additional Si and more Ca were released from Si-HA into medium. Comparing to pure HA nanrods and Ti substrate, Si-HA nanrods enhanced cell behaviors including proliferation, fibrotic phenotype and collagen secretion , and reduced epithelial down growth . The enhanced cell response and biosealing should be due to the releasing of Ca, Si and nanotopography of Si-HA nanorods. Si-HA nanorods can be a potential coating to accelerate skin integration for percutaneous implants in clinic.
理想的骨内经皮植入物除了在骨固定位置需要骨整合外，还应与软组织形成紧密密封。在钛上设计了释放硅离子并模拟天然组织纳米拓扑结构的硅取代羟基磷灰石（Si-HA）纳米棒，以增强成纤维细胞反应和与软组织的生物密封。通过碱热处理和水热处理制备了Si-HA纳米棒。探索了Si-HA纳米棒的水热形成机制。将Si-HA纳米棒的表面特性与纯HA纳米棒进行了比较。还评估了不同表面上的成纤维细胞行为和皮肤反应。所得结果表明，硅的取代并没有显著改变HA的相组成、形态、粗糙度和润湿性，但更多的硅和钙从Si-HA释放到培养基中。与纯HA纳米棒和钛基底相比，Si-HA纳米棒增强了细胞行为，包括增殖、纤维化表型和胶原蛋白分泌，并减少了上皮向下生长。细胞反应和生物密封的增强应该归因于Si-HA纳米棒中钙、硅的释放以及纳米拓扑结构。Si-HA纳米棒可能成为一种潜在的涂层，以加速临床经皮植入物的皮肤整合。
