Department of Food Science and Nutrition, College of Agricultural and Marine Sciences, Sultan Qaboos University, PO Box 34, Al-Koud-123, Muscat, Sultanate of Oman.
Exp Biol Med (Maywood). 2012 Sep;237(9):1018-22. doi: 10.1258/ebm.2012.012168. Epub 2012 Sep 6.
In a previous study, we demonstrated the ability of papaya epicarp extract (PEE) to protect against oxidative stress-induced insult in human SH-SY5Y neuronal cells in a mechanism that appeared to be by means of PEE potent antioxidant properties. To further understand this relationship, we examined the effect of PEE intervention on aluminum (Al)-induced cytotoxicity in SH-SY5Y cells. The results indicated that PEE was effective in protecting against Al-induced cell death in a dose-dependent manner. PEE restored the Al-induced inhibition of folate-dependent methionine synthase activity and the antioxidant enzymes (catalase, glutathione peroxidases and superoxide dismutase). PEE ameliorated the Al-induced impairment of intracellular glutathione and total antioxidant capacity. Together, these findings indicate that PEE supplementation can play a neuroprotective role in ameliorating the changes in redox status of SH-SY5Y cells exposed to Al, a well-known environmental toxin that is involved in the pathogenesis of neurodegenerative diseases and neurodevelopmental disorders.
在之前的研究中，我们证明了木瓜果皮提取物 (PEE) 具有保护人类 SH-SY5Y 神经元细胞免受氧化应激损伤的能力，其机制似乎是通过 PEE 强大的抗氧化特性。为了进一步了解这种关系，我们研究了 PEE 干预对 SH-SY5Y 细胞中铝 (Al) 诱导细胞毒性的影响。结果表明，PEE 能够以剂量依赖的方式有效抵抗 Al 诱导的细胞死亡。PEE 恢复了 Al 诱导的叶酸依赖性蛋氨酸合成酶活性和抗氧化酶（过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶）的抑制作用。PEE 改善了 Al 诱导的细胞内谷胱甘肽和总抗氧化能力的损伤。综上所述，这些发现表明，PEE 补充剂可以在改善暴露于 Al 的 SH-SY5Y 细胞氧化还原状态方面发挥神经保护作用，Al 是一种众所周知的环境毒素，与神经退行性疾病和神经发育障碍的发病机制有关。
