Bell Luke, Spadafora Natasha D, Müller Carsten T, Wagstaff Carol, Rogers Hilary J
Department of Food and Nutritional Sciences, University of Reading, Whiteknights, PO Box 226, Reading, Berkshire RG6 6AP, UK.
Cardiff School of Biosciences, Cardiff University, Main Building, PO Box 915, Cardiff, CF10 3TL, UK.
Food Chem. 2016 Mar 1;194:626-36. doi: 10.1016/j.foodchem.2015.08.043. Epub 2015 Aug 14.
An important step in breeding for nutritionally enhanced varieties is determining the effects of the post-harvest supply chain on phytochemicals and the changes in VOCs produced over time. TD-GC-TOF-MS was used and a technique for the extraction of VOCs from the headspace using portable tubes is described. Forty-two compounds were detected; 39 were identified by comparison to NIST libraries. Thirty-five compounds had not been previously reported in Eruca sativa. Seven accessions were assessed for changes in headspace VOCs over 7days. Relative amounts of VOCs across 3 time points were significantly different - isothiocyanate-containing molecules being abundant on 'Day 0'. Each accession showed differences in proportions/types of volatiles produced on each day. PCA revealed a separation of VOC profiles according to the day of sampling. Changes in VOC profiles over time could provide a tool for assessment of shelf life.
培育营养强化品种的一个重要步骤是确定收获后供应链对植物化学物质的影响以及随时间产生的挥发性有机化合物（VOCs）的变化。使用了热脱附-气相色谱-飞行时间质谱（TD-GC-TOF-MS），并描述了一种使用便携式采样管从顶空提取VOCs的技术。检测到42种化合物；通过与美国国家标准与技术研究院（NIST）数据库比对鉴定出39种。35种化合物此前未在芝麻菜中报道过。评估了7个种质在7天内顶空VOCs的变化。3个时间点的VOCs相对含量有显著差异——含异硫氰酸酯的分子在“第0天”含量丰富。每个种质在每天产生的挥发物比例/类型上都有差异。主成分分析（PCA）显示根据采样日期，VOCs谱图存在分离。VOCs谱图随时间的变化可为评估货架期提供一种工具。
